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Identification of transgelin as a potential novel biomarker for gastric adenocarcinoma based on proteomics technology

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Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript



To find a biomarker for gastric adenocarcinomas (GA).


Ten protein expression profiles of GA and paired non-neoplastic mucosa tissues were analyzed by two-dimensional gel electrophoresis. Forty-two protein spots that were differentially expressed by twofold or greater between cancer and normal mucosa tissue were excised and identified by MALDI-TOF/TOF MS. One of the over-expressed proteins identified in GA was transgelin, which was chosen for further verification by immunohistochemistry and western blotting.


Forty-two distinct proteins that were differentially expressed at least twofold between the tissues were identified. Expression of 29 of these proteins was decreased (ratio ≥ 2, P < 0.01), including adenosine deaminase; and 13 proteins displayed over-expression in cancer tissue (ratio ≥ 2, P < 0.01), including transgelin. The results of immunohistochemistry confirmed that transgelin was indeed over-expressed in 22 cases of GA (22/41, 53.66%), with strong cytoplasmic staining in cancer cells of positive samples, this was absent in most paired non-neoplastic mucosa cells or gastric ulcer tissues (n = 20). Transgelin was found over-expressed in 21 samples of cancer tissue (21/41, 51.22%) when detected by western blot.


This work demonstrates that differentially expressed proteins can be identified by proteomics technology combined with immunohistochemistry and western blot analyses. We have identified one such protein, transgelin, as a novel biomarker for GA.

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The authors are very grateful to Dr. Lie Wang and Zhiyong Zheng for offering the 41 cases of the GA and paired non-neoplastic tissue samples, Dr. Xin Ding for the pathological diagnosis and confirming the results of IHC, and Mr. Xisheng Xiong for technical assistance in the IHC.

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Correspondence to Xu Lin.

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Program for Innovation Research Team in Science and Technology in Fujian Province University FMU-RT001, Project of Science and Technology Development in Fujian Province 2005D087 and Professor Foundation of Fujian Medical University JS07003 (to Xu Lin).

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Huang, Q., Huang, Q., Chen, W. et al. Identification of transgelin as a potential novel biomarker for gastric adenocarcinoma based on proteomics technology. J Cancer Res Clin Oncol 134, 1219–1227 (2008).

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