Abstract
Objective
The purpose of this study is to identify single nucleotide polymorphisms (SNPs) in the coding region alleles of the X chromosomal LAGE-1 gene, and investigate the frequency of such SNPs in both cancer patients and healthy controls, and thus determine the potential significance of these SNPs with respect to cancer vaccine therapy.
Methods
In this study, different mRNAs transcribed from the LAGE-1 gene were identified by RT-PCR from healthy donors and cancer patients samples.
Results
A new LAGE-1 allele containing three coding region SNPs (69A/G, 317C/G, and 397T/G) were identified. The allele is highly expressed as the LAGE-1a mRNA variant AY679089 in some of the cancer patients. The three SNPs altered the LAGE-1 gene sequence to that of NYESO-1 at both the nucleotide and amino acid level.
Conclusion
There is a high frequency of the LAGE-1 gene allele with SNPs in coding regions in cancer patients. There was a clear relationship between the variant AY679089 and gastric cancer. The SNPs may lead to accelerated progress of poorly differentiated gastric cancer. The SNPs found in these alleles may also alter the immunological characteristics of LAGE-1a and should be taken into account if this antigen is adopted as a cancer vaccine component.
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Acknowledgments
This study was supported by Designed Grant of Cancer Research Institute (New York, NY), Hong Kong University of Science and Technology Grant Council, New Star Program of Science technology of the Beijing Science and Technology Committee (H020821370130), the Youth Foundation of Peking Union Medical College Hospital under Grant No. 2005 37A. National Natural Science Foundation of China (no.30600729) and China Medical Board In New York No.06-837.
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Yi Shao and Zhen-yuan Sun contributed equally to this article.
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Shao, Y., Sun, Zy., Sun, Sw. et al. Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients. J Cancer Res Clin Oncol 134, 495–502 (2008). https://doi.org/10.1007/s00432-007-0312-z
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DOI: https://doi.org/10.1007/s00432-007-0312-z