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SCC-112 gene is involved in tumor progression and promotes the cell proliferation in G2/M phase

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Abstract

Purpose

SCC-112 is a novel cell cycle-related gene and differentially expressed in cancers. Suggesting the complex role of SCC-112 might be existent in cell proliferation and tumor development. The relative research on SCC-112 has been few so far. This study is attempted to explore the role of SCC-112 in tumorigenesis.

Experimental design

RT-PCR and western blot were performed on seven tumor-normal paired tissues and nine cell lines. Immunohistochemistry was carried out for analyzing the expression of SCC-112 in nasopharyngeal tissues. 293T and three nasopharyngeal cell lines were transfected with expression vector (pCMV-SPORT6-SCC-112) or its siRNA. Cell proliferation was examined by MTT and clone formation experiments. Immunoprecipitation determined the interacted protein of SCC-112, and FACS detected cell cycle parameter on cells treated with synchronized reagent.

Results

SCC-112 (∼150 kDa) is up-regulated in tumor tissue as compared to the corresponding normal tissue and was detected in the tested cell lines. Overexpression of SCC-112 (∼150 kDa) in 293T and three nasopharyngeal cell lines promoted cell proliferation and clone formation while downregulation of SCC-112 (∼150 kDa) in these cells resulted in the opposite. Moreover, SCC-112 was found to interact with p63 and overexpression of SCC-112 up-regulated p63 expression. SCC-112 expression level positively correlated with cells in G2/M phase.

Conclusions

These findings suggest that SCC-112 improve cell proliferation and contributes to tumorigenesis by interacting with p63 and promoting cell cycling. SCC-112 might be an alternative target in tumor biomarking and mechanistic investigation.

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Acknowledgments

This work was supported by The National Natural Science Foundation of China (No. 30570817) and State Key Basic Research Programs 973 of China (NO.2002CB513101 and No.2004CB518701). We thank Ying Huang for collecting tissues and Zhong-yuan Tian for helping data management.

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Authors and Affiliations

  1. State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, People’s Republic of China

    M. Z. Zheng & Y. X. Zeng

  2. State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, People’s Republic of China

    L. M. Zheng

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  1. M. Z. Zheng
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  2. L. M. Zheng
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  3. Y. X. Zeng
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Correspondence to Y. X. Zeng.

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Zheng, M.Z., Zheng, L.M. & Zeng, Y.X. SCC-112 gene is involved in tumor progression and promotes the cell proliferation in G2/M phase. J Cancer Res Clin Oncol 134, 453–462 (2008). https://doi.org/10.1007/s00432-007-0306-x

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  • DOI: https://doi.org/10.1007/s00432-007-0306-x

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