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Journal of Cancer Research and Clinical Oncology

, Volume 133, Issue 11, pp 817–824 | Cite as

Highly aggressive behavior of malignant rhabdoid tumor: a special reference to SMARCB1/INI1 gene alterations using molecular genetic analysis including quantitative real-time PCR

  • Kenichi Kohashi
  • Yoshinao OdaEmail author
  • Hidetaka Yamamoto
  • Sadafumi Tamiya
  • Teiyu Izumi
  • Shigeru Ohta
  • Tomoaki Taguchi
  • Sachiyo Suita
  • Masazumi TsuneyoshiEmail author
Original Paper

Abstract

Purpose

SMARCB1/INI1, which negatively regulates cell cycle progression from G0/G1 into the S-phase via the p16INK4a-RB-E2F pathway, has been reported to be inactivated homozygously by deletion and/or mutations in malignant rhabdoid tumor (MRT). In the current study, we investigated the alteration of the SMARCB1/INI1 gene using simple methods, and its gene product at the protein level. Moreover, we investigated the status of hyperphosphorylation in RB protein, known as a key cell cycle molecule.

Methods

Three cell lines and 11 formalin-fixed, paraffin-embedded specimens of MRT were investigated. SMARCB1/INI1 gene alteration was analyzed with simple methods as a quantitative real-time PCR and direct sequencing method. Furthermore, SMARCB1/INI1 and RB protein were immunohistochemically evaluated.

Results

In 12 of 14 cases, we detected genetic alterations comprised of nine (including three cell lines) homozygous deletions and three mutations, which can induce abnormal expression of gene products. At the protein level, SMARCB1/INI1 immunohistochemical expressions were not detected in any cases. Twelve out of 14 cases showed high-level (+5) expression of tRB (both hyperphosphorylated and underphosphorylated RB), combined with low-level (+1) expression of uRB (underphosphorylated RB), indicating a high rate of hyperphosphorylation.

Conclusions

We could analyze the SMARCB1/INI1 gene alteration with simple methods, and SMARCB1/INI1 gene alteration was found in 12 of 14 cases. Especially, quantitative real-time PCR was a convenient and accurate method. In addition, a high rate of hyperphosphorylation of RB gene was recognized. These results suggest that the clinically aggressive character of MRT is caused by the inactivation of the SMARCB1/INI1 gene.

Keywords

Malignant rhabdoid tumor SMARCB1/INI1 RB Quantitative real-time PCR Immunohistochemistry 

Abbreviations

MRT

Malignant rhabdoid tumor

EMRT

Extrarenal malignant rhabdoid tumor

MRTK

Malignant rhabdoid tumor of the kidney

tRB

Both hyperphosphorylated and underphosphorylated RB

uRB

Underphosphorylated RB

Ct

Threshold cycle numbers

GDR

Gene dosage ratio

Notes

Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (C) (no. 18590332) from the Japan Society for the Promotion of Science, Tokyo, Japan. English used in this manuscript was revised by KN International (http://www.kninter.com/).

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Kenichi Kohashi
    • 1
  • Yoshinao Oda
    • 1
    Email author
  • Hidetaka Yamamoto
    • 1
  • Sadafumi Tamiya
    • 1
  • Teiyu Izumi
    • 1
  • Shigeru Ohta
    • 2
  • Tomoaki Taguchi
    • 3
  • Sachiyo Suita
    • 3
  • Masazumi Tsuneyoshi
    • 1
    Email author
  1. 1.Department of Anatomic Pathology, Pathological Sciences, Graduate School of Medical SciencesKyushu UniversityHigashi-ku, FukuokaJapan
  2. 2.Department of PediatricsShiga University of Medical Science, TsukinowaOhtsu, ShigaJapan
  3. 3.Department of Pediatric Surgery, Graduate School of Medical SciencesKyushu UniversityHigashi-ku, FukuokaJapan

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