Skip to main content

Advertisement

Log in

Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours

  • Original Paper
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Psoriasin (S100A7) has originally been described to be expressed by psoriatic keratinocytes possibly as a result of altered differentiation and inflammation. As psoriasin was found to be overexpressed in human breast and bladder cancer suggesting a role in tumour progression, we investigated the expression of psoriasin in human epithelial skin tumours.

Methods

Realtime reverse transcription-polymerase chain reaction experiments were performed to analyse the mRNA-expression levels of psoriasin together with involucrin as a marker for epithelial differentiation and interleukin-8 (IL-8) as a marker for inflammation in skin biopsy samples from patients with precancerous skin lesions (PSL, n = 6), squamous cell carcinoma (SCC, n = 11), basal cell carcinoma (BCC, n = 17), and healthy controls (n = 10).

Results

Unexpectedly, mRNA expression levels for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) revealed a variation of up to 600-fold in all cDNA-samples under investigation, indicating that GAPDH is not suitable as a housekeeping gene in human skin samples. Psoriasin mRNA expression was significantly up-regulated in samples of PSL, SCC and BCC. In situ hybridisation and immunohistochemical examinations identified psoriasin mRNA and protein expression in the differentiated layers of the epidermis. IL-8 mRNA expression was significantly up-regulated in SCC, however, there was no correlation between elevated levels of psoriasin and the expression of IL-8.

Conclusion

Similar to the findings in breast and bladder cancer, the up-regulation of psoriasin might play a role in the progression of skin cancer. The expression of psoriasin in human skin tumours seems to be independent from differentiation and inflammation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Algermissen B, Sitzmann J, LeMotte P, Czarnetzki B (1996) Differential expression of CRABP II, psoriasin and cytokeratin 1 mRNA in human skin diseases. Arch Dermatol Res 288:426–430

    PubMed  CAS  Google Scholar 

  • Al-Haddad S, Zhang Z, Leygue E, Snell L, Huang A, Niu Y, Hiller-Hitchcock T, Hole K, Murphy LC, Watson PH (1999) Psoriasin (S100A7) expression and invasive breast cancer. Am J Pathol 155:2057–2066

    PubMed  CAS  Google Scholar 

  • Alowami S, Qing G, Emberley E, Snell L, Watson PH (2003) Psoriasin (S100A7) expression is altered during skin tumorigenesis. BMC Dermatol 3:1

    Article  PubMed  Google Scholar 

  • Barker JN, Jones ML, Mitra RS, Crockett-Torabe E, Fantone JC, Kunkel SL, Warren JS, Dixit VM, Nickoloff BJ (1991) Modulation of keratinocyte-derived interleukin-8 which is chemotactic for neutrophils and T lymphocytes. Am J Pathol 139:869–876

    PubMed  CAS  Google Scholar 

  • Boni R, Schuster C, Nehrhoff B, Burg G (2002) Epidemiology of skin cancer. Neuro Endocrinol Lett 23(Suppl 2):48–51

    PubMed  Google Scholar 

  • Bornscheuer E, Schröder JM, Christophers E, Sticherling M (1996) Interleukin-8 immunoreactivity in malignant tumours of the skin. Acta Derm Venereol 76:210–213

    PubMed  CAS  Google Scholar 

  • Boukamp P (2005) Non-melanoma skin cancer: what drives tumor development and progression? Carcinogenesis 26:1657–1667

    Article  PubMed  CAS  Google Scholar 

  • Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169–193

    Article  PubMed  CAS  Google Scholar 

  • Carlsson H, Petersson S, Enerbäck C (2005) Cluster analysis of S100 gene expression and genes correlating to psoriasin (S100A7) expression at different stages of breast cancer development. Int J Oncol 27:1473–1481

    PubMed  CAS  Google Scholar 

  • Celis JE, Rasmussen HH, Vorum H, Madsen P, Honore B, Wolf H, Orntoft TF (1996) Bladder squamous cell carcinomas express psoriasin and externalize it to the urine. J Urol 155:2105–2112

    Article  PubMed  CAS  Google Scholar 

  • Di Nuzzo S, Sylva-Steenland RM, Koomen CW, de Rie MA, Das PK, Bos JD, Teunissen MB (2000) Exposure to UVB induces accumulation of LFA-1+ T cells and enhanced expression of the chemokine psoriasin in normal human skin. Photochem Photobiol 72:374–382

    Article  PubMed  CAS  Google Scholar 

  • Eckert RL, Broome AM, Ruse M, Robinson N, Ryan D, Lee K (2004) S100 proteins in the epidermis. J Invest Dermatol 123:23–33

    Article  PubMed  CAS  Google Scholar 

  • Emberley ED, Gietz RD, Campbell JD, HayGlass KT, Murphy LC, Watson PH (2002) RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer. BMC Cancer 2:28

    Article  PubMed  Google Scholar 

  • Emberley ED, Niu Y, Leygue E, Tomes L, Gietz RD, Murphy LC, Watson PH (2003) Psoriasin interacts with Jab1 and influences breast cancer progression. Cancer Res 63:1954–1961

    PubMed  CAS  Google Scholar 

  • Enerbäck C, Porter DA, Seth P, Sgroi D, Gaudet J, Weremowicz S, Morton CC, Schnitt S, Pitts RL, Stampl J, Barnhart K, Polyak K (2002) Psoriasin expression in mammary epithelial cells in vitro and in vivo. Cancer Res 62:43–47

    PubMed  Google Scholar 

  • Gläser R, Harder J, Lange H, Bartels J, Christophers E, Schröder JM (2005) Antimicrobial psoriasin (S100A7) protects human skin from Escherichia coli infection. Nat Immunol 6:57–64

    Article  PubMed  Google Scholar 

  • Hoffmann HJ, Olsen E, Etzerodt M, Madsen P, Thogersen HC, Kruse T, Celis JE (1994) Psoriasin binds calcium and is upregulated by calcium to levels that resemble those observed in normal skin. J Invest Dermatol 103:370–375

    Article  PubMed  CAS  Google Scholar 

  • Jinquan T, Vorum H, Larsen CG, Madsen P, Rasmussen HH, Gesser B, Etzerodt M, Honore B, Celis JE, Thestrup-Pedersen K (1996) Psoriasin: a novel chemotactic protein. J Invest Dermatol 107:5–10

    Article  PubMed  CAS  Google Scholar 

  • Krop I, Marz A, Carlsson H, Li X, Bloushtain-Qimron N, Hu M, Gelman R, Sabel MS, Schnitt S, Ramaswamy S, Kleer CG, Enerback C, Polyak K (2005) A putative role for psoriasin in breast tumor progression. Cancer Res 65:11326–11334

    Article  PubMed  CAS  Google Scholar 

  • La Placa M, Gibellini D, Bianchi T, Patrizi A (2006) Overexpression of MLH-1 and psoriasin genes in cutaneous angiofibromas from tuberous sclerosis complex patients. J Cutan Pathol 33:608–613

    Article  PubMed  Google Scholar 

  • Leygue E, Snell L, Hiller T, Dotzlaw H, Hole K, Murphy LC, Watson PH (1996) Differential expression of psoriasin messenger RNA between in situ and invasive human breast carcinoma. Cancer Res 56:4606–4609

    PubMed  CAS  Google Scholar 

  • Madan V, Hoban P, Strange RC, Fryer AA, Lear JT (2006) Genetics and risk factors for basal cell carcinoma. Br J Dermatol 154:5–7

    Article  PubMed  CAS  Google Scholar 

  • Madsen P, Rasmussen HH, Leffers H, Honore B, Dejgaard K, Olsen E, Kiil J, Walbum E, Andersen AH, Basse B, et al (1991) Molecular cloning, occurrence, and expression of a novel partially secreted protein psoriasin that is highly up-regulated in psoriatic skin. J Invest Dermatol 97:701–712

    Article  PubMed  CAS  Google Scholar 

  • Porter D, Lahti-Domenici J, Keshaviah A, Bae YK, Argani P, Marks J, Richardson A, Cooper A, Strausberg R, Riggins GJ, Schnitt S, Gabrielson E, Gelman R, Polyak K (2003) Molecular markers in ductal carcinoma in situ of the breast. Mol Cancer Res 1:362–375

    PubMed  CAS  Google Scholar 

  • Szepietowski JC, Walker C, McKenna DB, Hunter JA, McKenzie RC (2001) Leukaemia inhibitory factor and interleukin-8 expression in nonmelanoma skin cancers. Clin Exp Dermatol 26:72–78

    Article  PubMed  CAS  Google Scholar 

  • Watt FM (1983) Involucrin and other markers of keratinocyte terminal differentiation. J Invest Dermatol 81:100s–103s

    Article  PubMed  CAS  Google Scholar 

  • Wu YY, Rees JL (2000) Variation in epidermal housekeeping gene expression in different pathological states. Acta Derm Venereol 80:2–3

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank K. Klose (Department of Otorhinolaryngology, Kiel) for excellent technical assistance, the cutaneous surgery unit (Department of Dermatology, Kiel, and Praxis Dr. Dr. E. Paulke, Friedrichsort) for obtaining the skin biopsies and P. Velasco (Department of Dermatology, Kiel) for helpful discussions. This work was supported by the Schleswig-Holsteinische Krebsgesellschaft and the Deutsche Forschungsgemeinschaft (SFB 617).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Regine Gläser.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moubayed, N., Weichenthal, M., Harder, J. et al. Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours. J Cancer Res Clin Oncol 133, 253–261 (2007). https://doi.org/10.1007/s00432-006-0164-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00432-006-0164-y

Keywords

Navigation