Abstract
Purpose
Microarray analysis was used to bring a comprehensive insight into underlying molecular mechanisms and obtain a whole assessment of aberrant gene expression in nasopharyngeal carcinoma (NPC).
Methods
Combined with microdissection, gene expression profiles in 23 NPCs and 10 nontumor nasopharyngeal epithelial tissue samples were analyzed.
Results
Gene expression patterns suggested the dysregulation of the GTP/GDP-bound Ras cycle and an abnormal hyperactivity of cell cycle in NPC. Alterations in the WNT pathway suggest that this pathway may be activated in NPC. A 6-feature weighted-voting model was chosen because it represented the main characteristics of NPCs and predicted NPCs most accurately from the nontumor tissues (33 of 34 correct calls; 97.1% accuracy, Fisher’s exact test, P value = 8.389 × 10−8).
Conclusions
The data generated in this study represent a comprehensive list of genes aberrantly regulated in NPC. The 6-feature weighted-voting model may provide an extensive list of potential molecular markers for early diagnosis.
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Acknowledgment
This work was supported by New Century Excellent Talents in University (NCET-04-0761); National Natural Sciences Foundation of China, Grant numbers: 30330560, 30300201, 30200312, and 30470955; A Foundation for the Author of National Excellent Doctoral Dissertation of PR China (200559); the Special Fore Funds for Major State Basic Research of China (No. 2005 CCA 03200) and the Special Funds of Science & Technology Department of Hunan Province, China (05SK1001-1). The authors thank Dr. Yanjin Wang and Dr. Donghai Huang for guidance in microdissection of the nasopharyngeal epithelial tissues.
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Zhaoyang Zeng, Yanhong Zhou, Wei Xiong and Xiaomin Luo contributed equally to this work.
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Zeng, Z., Zhou, Y., Xiong, W. et al. Analysis of gene expression identifies candidate molecular markers in nasopharyngeal carcinoma using microdissection and cDNA microarray. J Cancer Res Clin Oncol 133, 71–81 (2007). https://doi.org/10.1007/s00432-006-0136-2
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DOI: https://doi.org/10.1007/s00432-006-0136-2