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A single nucleotide polymorphism in the MMP-1 promoter is correlated with histological differentiation of gastric cancer

Journal of Cancer Research and Clinical Oncology volume 130, pages 259–265 (2004)Cite this article

Abstract

Purpose

Matrix metalloproteinase-1 (MMP-1) plays a key role in cancer invasion and metastasis by degradation of extracellular matrix (ECM) and basement membrane barriers. The 1G/2G single nucleotide polymorphism (SNP) in the MMP-1 promoter at position –1607 bp has been reported to affect the transcriptional activity. In the light of these findings, we investigated whether this SNP in the MMP-1 promoter is associated with the development, differentiation, and progression of gastric cancer.

Methods

The 215 gastric cancer patients and 166 controls were used in this study. The SNP of the MMP-1 promoter was analyzed by PCR-RFLP and sequencing. The genotype frequency was compared between cases and controls, and the association with clinicopathological parameters among cases was studied.

Results

The frequency of 1G/2G genotypes in gastric cancer patients was similar to those in controls (p=0.57). The degree of tumor invasion, the presence of lymph node metastasis, and clinical stage showed no significant association with the SNP. On the other hand, we found a significant association with histological differentiation and gender among gastric cancer patients (p<0.05, respectively).

Conclusions

The presence of 2G allele in the MMP-1 promoter did not enhance the risk of gastric cancer; however, it may be involved in differentiation of gastric cancer.

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Affiliations

  1. Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, 734-8551, Hiroshima, Japan

    Shunji Matsumura, Naohide Oue & Wataru Yasui

  2. Department of Medicine and Molecular Science, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan

    Yasuhiko Kitadai & Kazuaki Chayama

  3. Department of Surgical Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan

    Kazuhiro Yoshida, Yoshiyuki Yamaguchi & Tetsuya Toge

  4. Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation, Hiroshima, Japan

    Kazue Imai & Kei Nakachi

Authors
  1. Shunji Matsumura
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  2. Naohide Oue
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  3. Yasuhiko Kitadai
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  4. Kazuaki Chayama
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  5. Kazuhiro Yoshida
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  6. Yoshiyuki Yamaguchi
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  7. Tetsuya Toge
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  8. Kazue Imai
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  9. Kei Nakachi
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  10. Wataru Yasui
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Corresponding author

Correspondence to Wataru Yasui.

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Matsumura, S., Oue, N., Kitadai, Y. et al. A single nucleotide polymorphism in the MMP-1 promoter is correlated with histological differentiation of gastric cancer. J Cancer Res Clin Oncol 130, 259–265 (2004). https://doi.org/10.1007/s00432-004-0543-1

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  • DOI: https://doi.org/10.1007/s00432-004-0543-1

Keywords

  • SNP
  • MMP-1
  • Gastric cancer