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Cloning, expression, and mutation analysis of NOR1, a novel human gene down-regulated in HNE1 nasopharyngeal carcinoma cell line

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Abstract

Purpose

To investigate cloning, expression, and mutation analysis of the putative candidate tumor suppressor gene related with nasopharyngeal carcinoma (NPC).

Methods

We studied the expression profiles in the NPC cell line HNE1 with the normal nasopharyngeal epithelial cell as control by using cDNA array representing 11,000 cDNA clusters. EST W95442 was found down-regulated in HNE1. Subsequently, the corresponding gene sequence including this EST was established by cDNA cloning and the RACE (rapid amplification of cDNA end) procedure. The expression pattern of this gene was examined by using Northern blot analysis in various human tissues. Furthermore, we screened the mutations of the coding sequence of the gene using reverse transcription-polymerase chain reaction and single-strand conformation polymorphisms (RT-PCR-SSCP) as well as direct sequencing analysis.

Results

A novel gene (GenBank accession No. AF462348) was cloned and named NOR1 standing for oxidored-nitro domain-containing protein 1 (Human Gene Nomenclature Committee-approved symbol). Northern blot analysis revealed that the NOR1 gene had two transcripts (1.2 kb, 1.6 kb), and expressed ubiquitously in human tissues. Moreover, a Glu58Gly mutation in the exon 1 of NOR1 was detected in two of 25 NPC biopsies.

Conclusions

We cloned a novel gene NOR1, and the Glu58Gly polymorphism of NOR1 may be involved in the development and/or progression of NPC suggesting that NOR1 could be a candidate tumor repressor gene related with NPC.

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Acknowledgements

This work is supported by (1) Chinese High Tech R&D Program, No.2001AA221031; (2) Chinese National Key Program on Basic Research, No.G1998051008; (3) National Natural Science Foundation of China, grants: 30100217.

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Authors and Affiliations

  1. Cancer Research Institute of Xiang-Ya School of Medicine, Central South University, 88 Xiangya Road, 410078, Changsha, Hunan, P.R. China

    Xinmin Nie, Bicheng Zhang, Xiaoling Li, Juanjuan Xiang, Bingyi Xiao, Jian Ma, Ming Zhou, Shiguo Zhu, Hongbin Lu & Guiyuan Li

  2. The 3rd Xiang-Ya Hospital of Central South University, Changsha, Hunan, P.R. China

    Xinmin Nie, Rong Gui & Shourong Shen

Authors
  1. Xinmin Nie
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  2. Bicheng Zhang
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  3. Xiaoling Li
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  4. Juanjuan Xiang
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  5. Bingyi Xiao
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  6. Jian Ma
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  7. Ming Zhou
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  8. Shiguo Zhu
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  9. Hongbin Lu
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  10. Rong Gui
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  11. Shourong Shen
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  12. Guiyuan Li
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Corresponding author

Correspondence to Guiyuan Li.

Additional information

This work is supported by: (1) Chinese High Tech R&D Program, No. 2001AA221031; (2) Chinese National Key Program on Basic Research, No. G1998051008; and (3) National Natural Science Foundation of China, grants 30100217

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Nie, X., Zhang, B., Li, X. et al. Cloning, expression, and mutation analysis of NOR1, a novel human gene down-regulated in HNE1 nasopharyngeal carcinoma cell line. J Cancer Res Clin Oncol 129, 410–414 (2003). https://doi.org/10.1007/s00432-003-0451-9

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  • DOI: https://doi.org/10.1007/s00432-003-0451-9

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