Abstract
The chromosome region 8p12-p22 shows frequent allelic loss in many neoplasms, including breast cancer (BC). The DLC-1 gene, located on 8p21-p22, might be a candidate tumor suppressor gene in this region. To evaluate the involvement of DLC-1 in breast carcinogenesis we studied DLC-1 mRNA expression in a panel of 14 primary human BC and the corresponding normal breast cells as well as 8 BC cell lines. Low levels or absence of DLC-1 mRNA were observed in 57% of primary BC and 62.5% of BC cell lines, respectively. We could not find any correlation between DLC-1mRNA expression and deletions at the DLC-1 locus. Transfection of the gene into DLC-1 deficient T-47D cells raised the DLC-1 mRNA level and resulted in inhibition of cell growth and reduced colony-forming capacity. Our results indicate a role of DLC-1 in BC carcinogenesis.
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Acknowledgements
We thank Sabine Werner for excellent technical assistance. This work was supported by the Deutsche Krebshilfe grant 70–2701.
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Plaumann, M., Seitz, S., Frege, R. et al. Analysis of DLC-1 expression in human breast cancer. J Cancer Res Clin Oncol 129, 349–354 (2003). https://doi.org/10.1007/s00432-003-0440-z
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DOI: https://doi.org/10.1007/s00432-003-0440-z