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Human kallikrein 2 (hK2) mRNA in peripheral blood of patients with thyroid cancer

A novel molecular marker?

  • Original Paper
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Purpose.

To elucidate whether hK2 mRNA can be detected in peripheral blood of patients with thyroid disease using reverse transcription polymerase chain reaction (RT-PCR).

Methods.

A nested RT-PCR protocol for the detection of hK2 mRNA was established, and blood samples of 72 patients with a history of thyroid cancer, 10 patients with current metastases of thyroid cancer, and 32 volunteers were tested.

Results.

hK2-transcripts were significantly more often detected in patients with thyroid cancer (20/72=28%) than in the control group (2/32=6%, P = 0.03, chi-square analysis).

Conclusions.

This is the first study reporting on hK2 as a potential molecular marker for patients with thyroid cancer. We could demonstrate a correlation between diagnosis of thyroid cancer and the positive signal for hK2 in the RT-PCR assay. Future studies are necessary to prove the clinical value of hK2 as a molecular marker regarding recurrence and outcome.

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Acknowledgments.

The authors are grateful to Simone Kräutlein and Carmen Knörnschild for their excellent technical assistance and to doctors and technicians of the Clinic for Nuclear Medicine, Offenbach for their help in collecting blood samples.

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Authors and Affiliations

  1. Dept. of Radiation Oncology, Academic Hospital, Starkenburgring 66, 63069, Offenbach, Germany

    Sandra J. Roddiger, Thomas Martin, Nikolaos Zamboglou & Ralf Kurek

  2. Dept. of Anatomy and Cell Biology, Philipps-University, Marburg, Germany

    Heiner Renneberg

  3. , Fred Hutchinson Cancer Research Center, Seattle, USA

    Heiner Renneberg

  4. Dept. of Urology, Academic Hospital, Offenbach, Germany

    Ulf W. Tunn

Authors
  1. Sandra J. Roddiger
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  2. Heiner Renneberg
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  3. Thomas Martin
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  4. Ulf W. Tunn
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  5. Nikolaos Zamboglou
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  6. Ralf Kurek
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Correspondence to Ralf Kurek.

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Roddiger, S.J., Renneberg, H., Martin, T. et al. Human kallikrein 2 (hK2) mRNA in peripheral blood of patients with thyroid cancer. J Cancer Res Clin Oncol 129, 29–34 (2003). https://doi.org/10.1007/s00432-002-0401-y

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  • DOI: https://doi.org/10.1007/s00432-002-0401-y

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