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Association between adropin and coronary artery lesions in children with Kawasaki disease

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Abstract

Kawasaki disease (KD) is an acute systemic vasculitis in children. Coronary artery lesions (CALs) are the most serious complications in KD, but the pathogenesis is still unclear so far. Adropin, a new biopeptide, plays an important role in metabolism and cardiovascular function. The aim of this study was to explore the relationship between adropin and KD. 66 KD patients and 22 healthy controls (HCs) were included in the study. KD patients were divided into KD with coronary artery lesions (KD-CALs) group and KD without CALs (KD-NCALs) group. The levels of serum adropin were determined by enzyme-linked immunosorbent assay (ELISA). Compared with the HC group, adropin concentrations were significantly increased in the KD group (p < 0.05), and the KD-CAL group had higher levels of adropin than those in the KD-NCAL group (p < 0.05). Pct (Procalcitonin) and DD (D-dimer) were positively correlated with adropin in the KD group (p < 0.05). Moreover, adropin had positive correlations with CRP (C-reactive protein) and DD in the KD-NCAL group and positive correlations with Pct, PLR (platelet-to-lymphocyte ratio), and DD in the KD-CAL group (p < 0.05). The receiver operating characteristic (ROC) curve showed that the best threshold value of serum adropin level was more than 2.8 ng/mL, with 72.2% sensitivity and 71.4% specificity for predicting CALs in children with KD.

Conclusion: Adropin might be involved in the pathogenesis of KD and CALs and can be used as an auxiliary diagnostic biomarker of KD.

What is Known:

• CALs in KD were mainly caused by inflammation, immune imbalance, and vascular endothelial dysfunction, and adropin is involved in metabolic diseases and cardiovascular diseases.

What is New:

• In this study, we have found the relationship between adropin and KD, and serum adropin level can be used as an auxiliary diagnostic biomarker to predict CALs in KD

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Data Availability

All datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

Abbreviations

AKT:

Protein Kinase B

AUC:

Area under the curve

APTT:

Activated partial thromboplastin time

BMI:

Body mass index

CRP:

C-reactive protein

CALs:

Coronary artery lesions

CAA:

Coronary artery aneurysm

CI:

Confidence interval

DD:

D-dimer

eNOS:

Endothelial NO synthase

ERK1/2:

Extracellular signal-regulated kinase 1/2

ELISA:

Enzyme-linked immunosorbent assay

ESR:

Erythrocyte sedimentation rate

Fbg:

Fibrinogen

HC:

Healthy control

KD:

Kawasaki disease

MPV:

Mean platelet volume

L%:

Percentage of leukomonocytes

N%:

Percentage of neutrophils

NLR:

Neutrophil-to-lymphocyte ratio

Pct:

Procalcitonin

PT:

Prothrombin time

Plt:

Platelet counts

PI3K:

Phosphatidylinositol 3-kinase

PPAR-γ:

Peroxisome proliferator-activated receptor γ

PLR:

Platelet-to-lymphocyte ratio

ROC:

Receiver operating characteristic

SPSS:

Statistical Package for Social Science

SD:

Standard deviations

TT:

Thrombin time

VEGFR2:

Vascular endothelial growth factor receptor-2

WBC:

White blood cell counts

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Funding

This research was supported by the National Natural Science Foundation of China (81270412).

Author information

Authors and Affiliations

Authors

Contributions

Maoling Yang: conceptualization, writing—original manuscript, investigation, writing—review and editing, software, and formal analysis. Qiongfei Pei: conceptualization and methodology. Jing Zhang: visualization and investigation. Haobo Weng: writing—reviewing and editing. Fengchuan Jing: writing—reviewing and editing. Qijian Yi: supervision, conceptualization, and writing—review and editing.

Corresponding author

Correspondence to Qijian Yi.

Ethics declarations

Ethics approval and consent to participate

Informed consent was obtained from the legal guardian of each patient. This research was approved by the ethics committee of Children’s Hospital of Chongqing Medical University. The study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as reflected in a priori approval by the institution's human research committee.

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Written informed consent for publication was obtained from all participants.

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All authors declare no conflict of interest.

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Communicated by Peter de Winter

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Yang, M., Pei, Q., Zhang, J. et al. Association between adropin and coronary artery lesions in children with Kawasaki disease. Eur J Pediatr 180, 2253–2259 (2021). https://doi.org/10.1007/s00431-021-03977-5

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  • DOI: https://doi.org/10.1007/s00431-021-03977-5

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