European Journal of Pediatrics

, Volume 168, Issue 11, pp 1305–1313 | Cite as

Mannose-binding lectin polymorphisms and recurrent respiratory tract infection in Chinese children

  • Jia Chen
  • Zhene Xu
  • Xi Ou
  • Mo Wang
  • Xiqiang Yang
  • Qiu LiEmail author
Original Paper


In order to establish the reference value of mannose-binding lectin (MBL) serum level in children and to investigate the correlation between the polymorphisms of MBL2 gene and serum MBL level in healthy Chinese of Han ethnic group and in children of Chinese Han ethnic group with recurrent respiratory tract infections (RRTI), the concentration of oligomerized MBL was measured by enzyme-linked immunosorbent assay, and MBL2 gene polymorphisms were analyzed by restriction fragment length polymorphism of polymerase chain reaction and polymerase chain reaction-sequence specific primer. The median MBL levels in the 470 normal children were 2536 ng/ml, and the P2.5–P97.5 was 161–5,070 ng/ml. Our research showed that two promoter polymorphisms at −550, −221 of start codon and coding variants at codon 54 of MBL2 gene affected the protein level significantly and the most frequent genotype in Hans is HYPA/HYPA. Our results also showed that serum MBL level was significantly lower in recurrent respiratory tract infections patients compared with healthy controls (Z, −3.04, P = 0.002). The frequency of the promoter LXP haplotype and the B allele was significantly higher in RRTI patients than in controls (χ 2 4.05, P < 0.05; OR 1.63, 95%CI 1.01∼2.62; χ 2 4.27, P < 0.05; OR 1.94, 95%CI 1.02∼3.68). Conclusion: We have established that the reference value of serum MBL level in Chinese aged between 0 and 6 years (161–5,070 ng/ml), and we found that LXP and the B are risk factors for RRTI.


Mannose-binding lectin Single nucleotide polymorphisms Recurrent respiratory tract infections 



Mannose-binding lectin


Sequence-specific primed polymerase chain reaction


Single nucleotide polymorphisms


Recurrent respiratory tract infections


Restriction fragment length polymorphism of polymerase chain reaction


Enzyme-linked immunosorbent assay



We thank the children who participated, their parents for their co-operation and interest in the study, and the National Natural Scientific Foundation of China.

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jia Chen
    • 1
  • Zhene Xu
    • 1
  • Xi Ou
    • 1
  • Mo Wang
    • 1
  • Xiqiang Yang
    • 1
  • Qiu Li
    • 1
    Email author
  1. 1.Department of Clinical Immunology, Children’s HospitalChongqing Medical UniversityChongqingChina

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