Abstract
The effects of perinatal problems on red cell phosphate metabolism were studied in two groups of infants (preterms B and fullterms D) during the first month of life. All infants started milk feeding from day three after birth. The results were compared to those of healthy preterms (A) and fullterms (C), respectively. Comparisons were also made between the preterm and fullterm groups B and D. The preterms with perinatal problems (B) showed a significant delay in catching up with the plasma and red cell inorganic phosphate (Pi) levels of controls (A) throughout the first month of life (p < 0.05). In parallel, the erythrocyte 2,3 diphosphoglycerate (2,3-DPG) concentrations of the sick preterms lagged significantly behind those of controls (p < 0.001); but the ATP levels were comparable between the two groups. The fullterms behaved slightly differently. No significant differences in plasma Pi (Pl Pi) and red cell 2,3-DPG were seen between the sick and healthy neonates during the month of study, while red cell Pi (RBC Pi) and ATP were found to be lower in the sick ones (p < 0.05). The fullterms with perinatal problems (D) had significantly higher Pl Pi (p < 0.05) and RBC Pi (p < 0.01) than preterms with problems (B) from the first week of life and continued in a similar pattern until the end of the month. Red cell 2,3-DPG concentrations were found to be significantly correlated with Pl Pi and RBC Pi in both preterm groups (p < 0.01) and in the sick fullterms (p < 0.001) during the time of the study. In the healthy fullterms 2,3-DPG was found to correlate only with red cell Pi (p < 0.05). Perinatal problems seem to affect Pi metabolism to a different degree in preterm and fullterm neonates in the first month of life.
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References
Adams H (1965) Adenosine 5’-triphosphate, determination with phosphoglycerate kinase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 539–543
Aladangady N, Coen PG, White MP, Rae MD, Beattie TJ (2004) Urinary excretion of calcium and phosphate in preterm infants. Pediatr Nephrol 19:1225–1231
Andronikou S, Rothberg AD, Pettifor JM, Thomson PD (1983) Early introduction of parenteral nutrition in premature infants and its effect on calcium and phosphate homeostasis. S Afr Med J 64:349–350
Bueva A, Guignard J-P (1994) Renal function in preterm neonates. Pediatr Res 36:572–577
Challa A, Bevington A, Anger CM, Asbury AJ, Preston CJ, Russell RGG (1985) A technique for the measurement of orthophosphate in human erythrocytes and some studies of its determinants. Clin Sci 69:429–434
Cholevas V, Challa A, Lapatsanis D, Andronikou S (1993) Phosphate metabolism in the red cell of sick prematures. Bone Miner 22:177–185
Delivoria-Papadopoulos M, Roncenic NP, Oski FA (1971) Postnatal changes in oxygen transport of term, premature and sick infants: the role of red cell 2,3 diphosphoglycerate and adult hemoglobin. Ped Res 5:235–245
Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Giapros V, Andronikou SK, Cholevas VI, Papadopoulou ZL (2003) Renal function and the effect of aminoglycoside therapy during the first days of life. Pediatr Nephrol 18:46–52
Giedion A, Haefliger H, Dangel P (1973) Acute pulmonary x-ray changes in hyaline membrane disease treated with artificial ventilation and positive end expiratory pressure. Pediat Radiol 1:145–152
Guignard JB, Ganyon JB (1988) Adverse effects of drugs on the immature kidney. Biol Neonate 53:243–252
Kalan G, Derganc M, Primozic J (2000) Phosphate metabolism in red cells of critically ill neonates. Pflugers Arch 440(5 Suppl):R109–R111
Lichtman MA, Miller DR, Cohen J, Waterhouse C (1971) Reduced red cell glycolysis, 2,3 diphosphoglycerate and adenoside triphosphate concentration and increased hemoglobin oxygen affinity caused by hypophosphatemia. Ann Intern Med 74:562–568
Oski FA, Gottlieb AJ, Miller WW, Delivoria-Papadopoulos M (1970) The effects of deoxygenation of adult and fetal hemoglobin in the synthesis of the red cell 2,3-DPG and its in vivo consequences. J Clin Invest 49:400–407
Rose ZB, Liebowitz J (1970) Direct determination of 2,3-diphosphoglycerate. Anal Biochem 35:177–183
Rowe JC, Carey DE (1987) Phosphorus deficiency syndrome in very low birth weight infants. Pediatr Clin N Am 34(4):997–1017
Sarnat H, Sarnat M (1976) Neonatal encephalopathy following fetal distress. Arch Neurol 33:696–705
Shaffer SE, Norman ME (1989) Renal function and renal failure in the newborn. Clin Perinatol 16:199–217
Siegel SR, Fisher DA, Oh W (1973) Renal function and serum aldosterone levels in infants with respiratory distress syndrome. J Pediatr 83:854–858
Travis SF, Sugerman HJ, Ruberg RL, Dudrick SJ, Delivoria-Papadopoulos M, Miller LD, Oski FA (1971) Alterations of red cell glycolytic intermediates and oxygen transport as a consequence of hypophosphatemia in patients receiving intravenous hyper-alimentation. N Engl J Med 285:763–768
Travis SF, Wagerle LC, De Alvarado CM, Rose G, Delivoria-Papadopoulos M (1985) Sequential changes in red cell glycolytic enzymes and intermediates and possible control mechanisms in the first two months of postnatal life in lambs. Pediatr Res 19(3):272–277
Tsirka A, Challa A, Lapatsanis PD (1990) Red cell phosphate metabolism in preterm infants with idiopathic respiratory distress syndrome. Acta Paed Scand 79:763–768
Tulassay T, Seri I (1986) Acute oliguria in preterm infants with hyaline membrane disease. Interaction of dopamin and furosemide. Acta Paed Scand 75:420–424
Vileisis RA (1987) Effect of phosphorus intake in total parenteral nutrition infusates in premature neonates. J Pediatr 110:586–590
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Cholevas, V., Challa, A., Lapatsanis, P.D. et al. Changes in red cell phosphate metabolism of preterm and fullterm infants with perinatal problems during their first month of life. Eur J Pediatr 167, 211–218 (2008). https://doi.org/10.1007/s00431-007-0464-5
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DOI: https://doi.org/10.1007/s00431-007-0464-5