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Association of IFIH1 and DDX58 genes polymorphism with susceptibility to COVID-19

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Abstract

Pattern recognition receptors of the innate immune system, such as RIG-I and MDA5, are responsible for recognizing viruses and inducing interferon production. Genetic polymorphisms in the coding regions of RLR may be associated with the severity of COVID-19. Considering the contribution of the RLR signaling in immune-mediated reactions, this study investigated the association between three SNP in the coding region of IFIH1 and DDX58 genes with the susceptibility to COVID-19 in the Kermanshah population, Iran. 177 patients with severe and 182 with mild COVID-19 were admitted for this study. Genomic DNA was extracted from peripheral blood leukocytes of patients to determine the genotypes of two SNPs, rs1990760(C>T) and rs3747517(T>C) IFIH1 gene and rs10813831(G>A) DDX58 gene using PCR–RFLP method. Our results showed that the frequency of the AA genotype of rs10813831(G>A) was associated with susceptibility to COVID-19 compared to the GG genotype (p = 0.017, OR = 2.593, 95% CI 1.173–5.736). We also observed a statistically significant difference in the recessive model for SNPs rs10813831 variant (AA versus GG + GA, p = 0.003, OR = 2.901, 95% CI 1.405–6.103). Furthermore, No significant association was found between rs1990760 (C>T) and rs3747517(T>C) of IFIH1 gene polymorphisms with COVID-19. Our findings suggest that DDX58 rs10813831(A>G) polymorphism may be associated with COVID-19 severity in the Kermanshah population, Iran.

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Data availability

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

MDA5:

Melanoma differentiation-associated protein 5

RIG-I:

Retinoic acid-inducible gene I

SARS-COV-2:

Severe acute respiratory syndrome coronavirus 2

IFN:

Interferon

RLR:

Retinoic acid-inducible gene-I (RIG-)-like receptors

NLR:

Nucleotide Oligomerization Domain (NOD)-like receptors

SNP:

Single nucleotide polymorphisms

ARDS:

Acute respiratory disease syndrome

PRR:

Pattern recognition receptors

IFIH1 :

Interferon-Induced Helicase 1

DDX58 :

DExD/H-box helicase 58

ARDS:

Acute respiratory distress syndrome

RFLP:

Polymerase chain reaction-restriction fragment length polymorphism

MAF:

Minor allele frequency

COVID-19:

Coronavirus disease-2019

OR:

Odds ratio

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Acknowledgements

We thank the deputy of Research and Technology of Kermanshah University of Medical Sciences for financial support.

Funding

This research has been supported by Grants from Kermanshah University of Medical Sciences (Grant number: 4010493).

Author information

Authors and Affiliations

  1. Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Daneshgah Street, Shahid Shiroudi Boulevard, PO-Box: 6714869914, Kermanshah, Iran

    Parisa Feizollahi, Sara Falahi, Farhad Salari, Ali Gorgin Karaji & Alireza Rezaiemanesh

  2. Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Hossein Zamanian

  3. Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Zahra Mahmoudi & Elham Faryadi

  4. Department of Immunology, School of Medicine, Tarbiat Modares University of Medical Sciences, Tehran, Iran

    Parisa Feizollahi

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Contributions

AR: Conceptualization, Visualization and Writing—Review and Editing. PF: Writing—Original draft preparation. AGK and FS: formal analysis and Validation. Pf and SF: Investigation and performing the laboratory experiments. ZM, EF and MHZ: resources and preparing the samples. AR critically revised the manuscript and provided the final approval. All authors read and approved the final manuscript and had full access to all the data in the study.

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Correspondence to Alireza Rezaiemanesh.

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Edited by Hanna-Mari Baldauf.

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Feizollahi, P., Zamanian, M.H., Falahi, S. et al. Association of IFIH1 and DDX58 genes polymorphism with susceptibility to COVID-19. Med Microbiol Immunol 212, 221–229 (2023). https://doi.org/10.1007/s00430-023-00764-x

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