Abstract
Novel strategies are needed for combating Staphylococcus aureus biofilm in vascular graft infections. We investigated the in vitro activity of bacteriophage endolysin HY-133, daptomycin and rifampin against S. aureus attached to vascular graft surface. Daptomycin showed rapid bactericidal effect on surface-associated S. aureus, while the activity of HY-133 on graft surface-adherent cells was moderate and rifampin did not achieve bactericidal effect. Even in the highest concentrations, all antimicrobials used failed in a complete eradication of the surface-adherent bacteria.
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Acknowledgements
We are grateful to Damayanti Kaiser for expert technical assistance and Ulrike Keller (Institute of Medical Physics and Biophysics, University of Münster) for support with the SEM.
Funding
This research was supported by the fund: “Innovative Medical Research” from Medical School of the University of Münster (ID 111317) and partly by the BMBF-DZIF (German Center for Infection Research, TTU 08.807 HAARBI, 8037808809 and 8037808907). Vascular graft material was provided by B. Braun, Melsungen, Germany (Uni-Graft® K DV).
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Figure S1 Killing kinetics of antimicrobials against vascular graft surface-adherent S. aureus SH1000 after 4-h and 18-h incubation; quantified by the ATP measurement (JPG 528 kb)
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Idelevich, E.A., Knaack, D., Nugroho, N.T. et al. Comparative in vitro activity of bacteriophage endolysin HY-133 against Staphylococcus aureus attached to vascular graft surface. Med Microbiol Immunol 209, 51–57 (2020). https://doi.org/10.1007/s00430-019-00638-1
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DOI: https://doi.org/10.1007/s00430-019-00638-1