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Biogenic Au@ZnO core–shell nanocomposites kill Staphylococcus aureus without provoking nuclear damage and cytotoxicity in mouse fibroblasts cells under hyperglycemic condition with enhanced wound healing proficiency

Medical Microbiology and Immunology volume 208, pages 609–629 (2019)Cite this article

Abstract

The aim of the present study is focused on the synthesis of Au@ZnO core–shell nanocomposites, where zinc oxide is overlaid on biogenic gold nanoparticles obtained from Hibiscus Sabdariffa plant extract. Optical property of nanocomposites is investigated using UV–visible spectroscopy and crystal structure has been determined using X-ray crystallography (XRD) technique. The presence of functional groups on the surface of Au@ZnO core–shell nanocomposites has been observed by Fourier transforms infrared (FTIR) spectroscopy. Electron microscopy studies revealed the morphology of the above core–shell nanocomposites. The synthesized nanocomposite material has shown antimicrobial and anti-biofilm activity against Staphylococcus aureus and Methicillin Resistant Staphylococcus haemolyticus (MRSH). The microbes are notorious cross contaminant and are known to cause infection in open wounds. The possible antimicrobial mechanism of as synthesized nanomaterials has been investigated against Staphylococcus aureus and obtained data suggests that the antimicrobial activity could be due to release of reactive oxygen species (ROS). Present study has revealed that surface varnishing of biosynthesized gold nanoparticles through zinc oxide has improved its antibacterial proficiency against Staphylococcus aureus, whereas reducing its toxic effect towards mouse fibroblast cells under normal and hyperglycaemic condition. Further studies have been performed in mice model to understand the wound healing efficiency of Au@ZnO nanocomposites. The results obtained suggest the possible and effective use of as synthesized core shell nanocomposites in wound healing.

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Acknowledgements

This work is supported by Department of Biotechnology (DBT), Government of India (Grant No. BT/AB/08/01/2008-III). Md. Imran Khan is thankful to University Grant Commission, New Delhi for supporting him through UGC-MANF programme.

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Affiliations

  1. School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Bhubaneswar, Odisha, 751024, India

    Md. Imran Khan, Prajita Paul, Mrutyunjay Suar, Suraj K. Tripathy & Amrita Mishra

  2. IMGENEX India Pvt. Ltd., Bhubaneswar, India

    Susanta Kumar Behera

  3. Department of Life Sciences, National Institute of Technology, Rourkela, Odisha, 769008, India

    Bhaskar Das & R. Jayabalan

  4. Murdoch Applied Nanotechnology Research Group, Department of Physics, Energy Studies and Nanotechnology, School of Engineering and Energy, Murdoch University, Murdoch, WA, Australia

    Derek Fawcett & Gerrard Eddy Jai Poinern

  5. School of Chemical Technology, Kalinga Institute of Industrial Technology (KIIT), Bhubaneswar, 751024, Odisha, India

    Suraj K. Tripathy

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  1. Md. Imran Khan
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Khan, M.I., Behera, S.K., Paul, P. et al. Biogenic Au@ZnO core–shell nanocomposites kill Staphylococcus aureus without provoking nuclear damage and cytotoxicity in mouse fibroblasts cells under hyperglycemic condition with enhanced wound healing proficiency. Med Microbiol Immunol 208, 609–629 (2019). https://doi.org/10.1007/s00430-018-0564-z

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Keywords

  • Biofilm
  • Core–shell
  • Gold
  • Staphylococcus aureus
  • Methicillin resistant Staphylococcus haemolyticus
  • Zinc oxide