Medical Microbiology and Immunology

, Volume 207, Issue 3–4, pp 201–210 | Cite as

Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran

  • Mahsa Farsi
  • Fatemeh Roodbari
  • Behrooz Nejati
  • Arash Arashkia
  • Somayeh Jalilvand
  • Alireza Nateghian
  • Aliakbar Rahbarimanesh
  • Sayed Mahdi Marashi
  • Zabihollah Shoja
Original Investigation


Viral gastroenteritis is a major public health problem worldwide. In Iran, very limited studies have been performed with regard to the epidemiology of noroviruses. This study aimed to evaluate the prevalence and molecular epidemiology of GII noroviruses in hospitalized children less than 5 years of age with acute gastroenteritis (AGE). A total of 210 stool specimens were collected from Ali Asghar Children’s Hospital and Bahrami Children’s Hospital in Tehran, from June 2015 to June 2016. The samples were screened by real-time RT-PCR for genogroup II (GII). Positive samples were genotyped by semi-nested PCR followed by Sanger sequencing and phylogenetic analysis. Norovirus was identified in 36 (17.1%) of 210 specimens. Based on genetic analysis of RdRp and capsid sequences, the strains were clustered into eight RdRp–capsid genotypes: GII.P4–GII.4 Sydney_2012 (41.7%), GII.Pe–GII.4 Sydney_2012 (30.6%), GII.P21–GII.3 (13.9%), GII.P16–GII.4 Sydney_2012 (2.8%), GII.P16–GII.12 (2.8%), GII.P2–GII.4 Sydney_2012 (2.8%), GII.P7–GII.7 (2.8%) and GII.P2–GII.2 (2.8%). We determined several different co-circulating norovirus genotypes in children < 5 years of age with AGE in our hospital in Tehran, Iran. Continued molecular surveillance of noroviruses, including typing of both RdRp and capsid genes, is important for monitoring emerging strains in our continued efforts to reduce the overall burden of norovirus disease.


Norovirus Gastroenteritis Genotyping RdRp Capsid 



We thank Ali Asghar and Bahrami Children’s Hospitals in Tehran for providing clinical samples.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicting financial or other interests.


  1. 1.
    Green KY (2013) Caliciviridae: the noroviruses. In: Howley DMKAP (ed) Fields virology. Lippincott Williams &Wilkins, Philadelphia, pp 582–608Google Scholar
  2. 2.
    Patel MM, Widdowson MA, Glass RI, Akazawa K, Vinje J, Parashar UD (2008) Systematic literature review of role of noroviruses in sporadic gastroenteritis. Emerg Infect Dis 14:1224–1231CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Payne DC, Vinje J, Szilagyi PG, Edwards KM, Staat MA, Weinberg GA et al (2013) Norovirus and medically attended gastroenteritis in US children. N Engl J Med 368:1121–1130CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Ahmed SM, Hall AJ, Robinson AE, Verhoef L, Premkumar P, Parashar UD et al (2014) Global prevalence of norovirus in cases of gastroenteritis: a systematic review and meta-analysis. Lancet Infect Dis 14:725–730CrossRefPubMedGoogle Scholar
  5. 5.
    Kapikian AZ, Wyatt RG, Dolin R, Thornhill TS, Kalica AR, Chanock RM (1972) Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol 10:1075–1081PubMedPubMedCentralGoogle Scholar
  6. 6.
    Karst SM, Wobus CE, Goodfellow IG, Green KY, Virgin HW, (2014) Advances in norovirus biology. Cell Host Microbe 15:668–680CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Vinje J, (2015) Advances in laboratory methods for detection and typing of norovirus. J Clin Microbiol 53:373–381CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Kroneman A, Vega E, Vennema H, Vinjé J, White PA, Hansman G et al (2013) Proposal for a unified norovirus nomenclature and genotyping. Arch Virol 158:2059–2068CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Vega E, Barclay L, Gregoricus N, Shirley SH, Lee D, Vinje J (2014) Genotypic and epidemiologic trends of norovirus outbreaks in the United States, 2009 to 2013. J Clin Microbiol 52:147–155CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Zheng DP, Widdowson MA, Glass RI, Vinje J (2010) Molecular epidemiology of genogroup II-genotype 4 noroviruses in the United States between 1994 and 2006. J Clin Microbiol 48:168–177CrossRefPubMedGoogle Scholar
  11. 11.
    Siebenga JJ, Vennema H, Zheng DP, Vinje J, Lee BE, Pang XL et al (2009) Norovirus illness is a global problem: emergence and spread of norovirus GII.4 variants, 2001–2007. J Infect Dis 200:802–812CrossRefPubMedGoogle Scholar
  12. 12.
    Kroneman A, Vennema H, Deforche K, v d Avoort H, Penaranda S, Oberste MS et al (2011) An automated genotyping tool for enteroviruses and noroviruses. J Clin Virol 51:121–125CrossRefPubMedGoogle Scholar
  13. 13.
    Centers for Disease Control and Prevention (2018) Number of confirmed norovirus outbreaks submitted to CaliciNet by state September 1, 2017–February 28, 2018.
  14. 14.
    Lindesmith LC, Brewer-Jensen PD, Mallory ML, Debbink K, Swann EW, Vinje J et al (2018) Antigenic characterization of a novel recombinant GII.P16-GII.4 Sydney norovirus strain with minor sequence variation leading to antibody escape. J Infect Dis 217:1145–1152CrossRefPubMedGoogle Scholar
  15. 15.
    Fazeli Z, Baghaie N, Khavarinejad RA, Khoramdel M, Sigaroodi A, Nadji SA (2010) Hospital based study of prevalence and genotyping of Noroviruses and Sapoviruses isolated from children with acute gastroenteritis referred to Masih Daneshvari hospital. Gastroenterol Hepatol Bed Bench 3:91–97Google Scholar
  16. 16.
    Jalilian S, Samarbaf-Zadeh AR, Mozhgani SHR, Makvandi M, Parsa-nahad M (2012) Relative frequency of norovirus infection in children suffering from gastroenteritis referred to Aboozar hospital, Ahvaz, Iran. Jundishapur J Microbiol 5:355–358Google Scholar
  17. 17.
    Najafi A, Vahdat K, Kargar M, Javdanic N (2013) Importance of viral pathogens in children with acute gastroenteritis in the south of Iran. Ann Saudi Med 33:124CrossRefPubMedGoogle Scholar
  18. 18.
    Romani S, Mohebbi SR, Hosseini SM, Azimzadeh P, Vahedi M, Derakhshan F et al (2012) Prevalence of norovirus infection in children and adults with acute gastroenteritis, Tehran, Iran, 2008–2009. Food Environ Virol 4:1–5CrossRefPubMedGoogle Scholar
  19. 19.
    Sharifi-Rad J, Alfatemi SMH, Sharifi-Rad M, Miri A (2015) Frequency of adenoviruses, rotaviruses and noroviruses among diarrhea samples collected from infants of Zabol, southeastern Iran. Jundishapur J Microbiol 8:e15440CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Roodsari SR, Bitajian F, Gachkar L, Jadali F, Adabian S, Nia RSS et al (2013) Detection of noroviruses isolated from children with acute gastroenteritis by Rt-PCR in Iran. Arch Pediatr Infect Dis 1:57–60CrossRefGoogle Scholar
  21. 21.
    Shoja Z, Jalilvand S, Mollaei-Kandelous Y, Validi M (2014) Epidemiology of viral gastroenteritis in Iran. Pediatr Infect Dis J 33:218–220CrossRefPubMedGoogle Scholar
  22. 22.
    Kreidieh K, Charide R, Dbaibo G, Melhem NM (2017) The epidemiology of norovirus in the Middle East and North Africa (MENA) region: a systematic review. Virol J 14:220CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Hoa Tran TN, Trainor E, Nakagomi T, Cunliffe NA, Nakagomi O (2013) Molecular epidemiology of noroviruses associated with acute sporadic gastroenteritis in children: global distribution of genogroups, genotypes and GII.4 variants. J Clin Virol 56:185–193CrossRefPubMedGoogle Scholar
  24. 24.
    Kaplan NM, Kirby A, Abd-Eldayem SA, Dove W, Nakagomi T, Nakagomi O et al (2011) Detection and molecular characterisation of rotavirus and norovirus infections in Jordanian children with acute gastroenteritis. Arch Virol 156:1477–1480CrossRefPubMedGoogle Scholar
  25. 25.
    Ayouni S, Estienney M, Sdiri-Loulizi K, Ambert-Balay K, de Rougemont A, Aho S et al (2015) Relationship between GII.3 norovirus infections and blood group antigens in young children in Tunisia. Clin Microbiol Infect 21:874 e1-8CrossRefPubMedGoogle Scholar
  26. 26.
    Najafi A, Najafi S, Vahdat K, Kargar M, Javdani N (2013) Importance of viral pathogens in children with acute gastroenteritis in the south of Iran. Ann Saudi Med 33:124–129CrossRefPubMedGoogle Scholar
  27. 27.
    Rolfe KJ, Parmar S, Mururi D, Wreghitt TG, Jalal H, Zhang H et al (2007) An internally controlled, one-step, real-time RT-PCR assay for norovirus detection and genogrouping. J Clin Virol 39:318–321CrossRefPubMedGoogle Scholar
  28. 28.
    Kageyama T, Kojima S, Shinohara M, Uchida K, Fukushi S, Hoshino FB et al (2003) Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J Clin Microbiol 41:1548–1557CrossRefPubMedPubMedCentralGoogle Scholar
  29. 29.
    Vennema H, de Bruin E, Koopmans M (2002) Rational optimization of generic primers used for Norwalk-like virus detection by reverse transcriptase polymerase chain reaction. J Clin Virol 25:233–235CrossRefPubMedGoogle Scholar
  30. 30.
    Vinje J, andKoopmans MP (1996) Molecular detection and epidemiology of small round-structured viruses in outbreaks of gastroenteritis in The Netherlands. J Infect Dis 174:610–615CrossRefPubMedGoogle Scholar
  31. 31.
    Kojima S, Kageyama T, Fukushi S, Hoshino FB, Shinohara M, Uchida K et al (2002) Genogroup-specific PCR primers for detection of Norwalk-like viruses. J Virol Methods 100:107–114CrossRefPubMedGoogle Scholar
  32. 32.
    Yuen LK, Catton MG, Cox BJ, Wright PJ, Marshall JA (2001) Heminested multiplex reverse transcription-PCR for detection and differentiation of Norwalk-like virus genogroups 1 and 2 in fecal samples. J Clin Microbiol 39:2690–2694CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Bruggink LD, Oluwatoyin O, Sameer R, Witlox KJ, Marshall JA (2012) Molecular and epidemiological features of gastroenteritis outbreaks involving genogroup I norovirus in Victoria, Australia, 2002–2010. J Med Virol 84:1437–1448CrossRefPubMedGoogle Scholar
  34. 34.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680CrossRefPubMedPubMedCentralGoogle Scholar
  35. 35.
    Jukes TH, (1969) Evolutionary pattern of specificity regions in light chains of immunoglobulins. Biochem Genet 3:109–117CrossRefPubMedGoogle Scholar
  36. 36.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Boga JA, Melon S, Nicieza I, De Diego I, Villar M, Parra F et al (2004) Etiology of sporadic cases of pediatric acute gastroenteritis in asturias, Spain, and genotyping and characterization of norovirus strains involved. J Clin Microbiol 42:2668–2674CrossRefPubMedPubMedCentralGoogle Scholar
  38. 38.
    Buesa J, Collado B, Lopez-Andujar P, Abu-Mallouh R, Rodriguez Diaz J, Garcia Diaz A et al (2002) Molecular epidemiology of caliciviruses causing outbreaks and sporadic cases of acute gastroenteritis in Spain. J Clin Microbiol 40:2854–2859CrossRefPubMedPubMedCentralGoogle Scholar
  39. 39.
    Shioda K, Kambhampati A, Hall AJ, Lopman BA (2015) Global age distribution of pediatric norovirus cases. Vaccine 33:4065–4068CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    Ahmed SM, Lopman BA, Levy K (2013) A systematic review and meta-analysis of the global seasonality of norovirus. PLoS One 8:e75922CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Boon D, Mahar JE, Abente EJ, Kirkwood CD, Purcell RH, Kapikian AZ et al (2011) Comparative evolution of GII.3 and GII.4 norovirus over a 31-year period. J Virol 85:8656–8666CrossRefPubMedPubMedCentralGoogle Scholar
  42. 42.
    Cho HG, Lee SG, Kim JE, Yu KS, Lee DY, Park PH et al (2014) Molecular epidemiology of norovirus GII.4 variants in children under 5 years with sporadic acute gastroenteritis in South Korea during 2006–2013. J Clin Virol 61:340–344CrossRefPubMedGoogle Scholar
  43. 43.
    Yu Y, Yan S, Li B, Pan Y, Wang Y (2014) Genetic diversity and distribution of human norovirus in China (1999–2011). Biomed Res Int 2014:196169PubMedPubMedCentralGoogle Scholar
  44. 44.
    Fu JG, Ai J, Zhang J, Wu QB, Qi X, Ji H et al (2016) Molecular epidemiology of genogroup II norovirus infection among hospitalized children with acute gastroenteritis in Suzhou (Jiangsu, China) from 2010 to 2013. J Med Virol 88:954–960CrossRefPubMedGoogle Scholar
  45. 45.
    Al-Mashhadani MN, Nakagomi O, Dove W, Ahmed H, Nakagomi T, Hart CA et al (2008) Norovirus gastroenteritis among children in Iraqi Kurdistan. J Med Virol 80:506–509CrossRefPubMedGoogle Scholar
  46. 46.
    Bucardo F, Reyes Y, Becker-Dreps S, Bowman N, Gruber JF, Vinje J et al (2017) Pediatric norovirus GII.4 infections in Nicaragua, 1999–2015. Infect Genet Evol 55:305–312CrossRefPubMedPubMedCentralGoogle Scholar
  47. 47.
    Estevez A, Arvelo W, Hall AJ, Lopez MR, Lopez B, Reyes L et al (2013) Prevalence and genetic diversity of norovirus among patients with acute diarrhea in Guatemala. J Med Virol 85:1293–1298CrossRefPubMedPubMedCentralGoogle Scholar
  48. 48.
    Huhti L, Blazevic V, Puustinen L, Hemming M, Salminen M, Vesikari T (2014) Genetic analyses of norovirus GII.4 variants in Finnish children from 1998 to 2013. Infect Genet Evol 26:65–71CrossRefPubMedGoogle Scholar
  49. 49.
    Kittigul L, Pombubpa K, Taweekate Y, Diraphat P, Sujirarat D, Khamrin P et al (2010) Norovirus GII-4 2006b variant circulating in patients with acute gastroenteritis in Thailand during a 2006–2007 study. J Med Virol 82:854–860CrossRefPubMedGoogle Scholar
  50. 50.
    Leshem E, Givon-Lavi N, Vinje J, Gregoricus N, Parashar U, Dagan R (2015) Differences in norovirus-associated hospital visits between Jewish and Bedouin children in Southern Israel. Pediatr Infect Dis J 34:1036–1038CrossRefPubMedGoogle Scholar
  51. 51.
    Victoria M, Carvalho-Costa FA, Heinemann MB, Leite JP, Miagostovich M (2007) Prevalence and molecular epidemiology of noroviruses in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil, 2004. Pediatr Infect Dis J 26:602–606CrossRefPubMedGoogle Scholar
  52. 52.
    Martella V, Medici MC, De Grazia S, Tummolo F, Calderaro A, Bonura F et al (2013) Evidence for recombination between pandemic GII.4 norovirus strains New Orleans 2009 and Sydney 2012. J Clin Microbiol 51:3855–3857CrossRefPubMedPubMedCentralGoogle Scholar
  53. 53.
    Eden JS, Hewitt J, Lim KL, Boni MF, Merif J, Greening G et al (2014) The emergence and evolution of the novel epidemic norovirus GII.4 variant Sydney 2012. Virology 450–451:106–113CrossRefPubMedGoogle Scholar
  54. 54.
    Fonager J, Barzinci S, Fischer TK (2013) Emergence of a new recombinant Sydney 2012 norovirus variant in Denmark, 26 December 2012 to 22 March 2013. Euro Surveill 18:20506CrossRefPubMedGoogle Scholar
  55. 55.
    van Beek J, Ambert-Balay K, Botteldoorn N, Eden JS, Fonager J, Hewitt J et al (2013) Indications for worldwide increased norovirus activity associated with emergence of a new variant of genotype II.4, late 2012. Euro Surveill 18:8–9PubMedGoogle Scholar
  56. 56.
    Giammanco GM, De Grazia S, Tummolo F, Bonura F, Calderaro A, Buonavoglia A et al (2013) Norovirus GII.4/Sydney/2012 in Italy, winter 2012–2013. Emerg Infect Dis 19:1348–1349CrossRefPubMedPubMedCentralGoogle Scholar
  57. 57.
    Shen Z, Qian F, Li Y, Hu Y, Yuan Z, Zhang J (2013) Novel norovirus GII.4 variant, Shanghai, China, 2012. Emerg Infect Dis 19:1337–1339CrossRefPubMedPubMedCentralGoogle Scholar
  58. 58.
    Rahman M, Nahar S, Afrad MH, Faruque AS, Azim T (2013) Norovirus variant GII.4/Sydney/2012, Bangladesh. Emerg Infect Dis 19:1347–1348PubMedPubMedCentralGoogle Scholar
  59. 59.
    Chan MC, Chan PK (2013) Complete genome sequence of a novel recombinant human norovirus Genogroup II genotype 4 strain associated with an epidemic during summer of 2012 in Hong Kong. Genome Announc 1:e00140-12CrossRefPubMedPubMedCentralGoogle Scholar
  60. 60.
    Cannon JL, Barclay L, Collins NR, Wikswo ME, Castro CJ, Magana LC et al (2017) Genetic and epidemiologic trends of norovirus outbreaks in the United States from 2013 to 2016 Demonstrated Emergence of Novel GII.4 Recombinant Viruses. J Clin Microbiol 55:2208–2221CrossRefPubMedPubMedCentralGoogle Scholar
  61. 61.
    White PA (2014) Evolution of norovirus. Clin Microbiol Infect 20:741–745CrossRefPubMedGoogle Scholar
  62. 62.
    Chhabra P, Dhongade RK, Kalrao VR, Bavdekar AR, Chitambar SD (2009) Epidemiological, clinical, and molecular features of norovirus infections in western India. J Med Virol 81:922–932CrossRefPubMedGoogle Scholar
  63. 63.
    Mahar JE, Bok K, Green KY, Kirkwood CD (2013) The importance of intergenic recombination in norovirus GII.3 evolution. J Virol 87:3687–3698CrossRefPubMedPubMedCentralGoogle Scholar
  64. 64.
    Phan TG, Kaneshi K, Ueda Y, Nakaya S, Nishimura S, Yamamoto A et al (2007) Genetic heterogeneity, evolution, and recombination in noroviruses. J Med Virol 79:1388–1400CrossRefPubMedGoogle Scholar
  65. 65.
    Wangchuk S, Matsumoto T, Iha H, Ahmed K (2017) Surveillance of norovirus among children with diarrhea in four major hospitals in Bhutan: replacement of GII.21 by GII.3 as a dominant genotype. PLoS One 12:e0184826CrossRefPubMedPubMedCentralGoogle Scholar
  66. 66.
    Bok K, Abente EJ, Realpe-Quintero M, Mitra T, Sosnovtsev SV, Kapikian AZ et al (2009) Evolutionary dynamics of GII.4 noroviruses over a 34-year period. J Virol 83:11890–11901CrossRefPubMedPubMedCentralGoogle Scholar
  67. 67.
    Bruggink LD, andMarshall JA (2009) Molecular and epidemiological features of GIIb norovirus outbreaks in Victoria, Australia, 2002–2005. J Med Virol 81:1652–1660CrossRefPubMedGoogle Scholar
  68. 68.
    Bull RA, Hansman GS, Clancy LE, Tanaka MM, Rawlinson WD, White PA (2005) Norovirus recombination in ORF1/ORF2 overlap. Emerg Infect Dis 11:1079–1085CrossRefPubMedPubMedCentralGoogle Scholar
  69. 69.
    Mans J, Murray TY, Nadan S, Netshikweta R, Page NA, Taylor MB (2016) Norovirus diversity in children with gastroenteritis in South Africa from 2009 to 2013: GII.4 variants and recombinant strains predominate. Epidemiol Infect 144:907–916CrossRefPubMedGoogle Scholar
  70. 70.
    Supadej K, Khamrin P, Kumthip K, Kochjan P, Yodmeeklin A, Ushijima H et al (2017) Wide variety of recombinant strains of norovirus GII in pediatric patients hospitalized with acute gastroenteritis in Thailand during 2005 to 2015. Infect Genet Evol 52:44–51CrossRefPubMedGoogle Scholar
  71. 71.
    Phumpholsup T, Chieochansin T, Vongpunsawad S, Vuthitanachot V, Payungporn S, Poovorawan Y (2015) Human norovirus genogroup II recombinants in Thailand, 2009–2014. Arch Virol 160:2603–2609CrossRefPubMedGoogle Scholar
  72. 72.
    Zhirakovskaia EV, Tikunov AY, Bodnev SA, Klemesheva VV, Netesov SV, Tikunova NV (2015) Molecular epidemiology of noroviruses associated with sporadic gastroenteritis in children in Novosibirsk, Russia, 2003–2012. J Med Virol 87:740–753CrossRefPubMedGoogle Scholar
  73. 73.
    Fumian TM, da Silva Ribeiro de Andrade J, Leite JP, Miagostovich MP (2016) Norovirus recombinant strains isolated from Gastroenteritis outbreaks in Southern Brazil, 2004–2011. PLoS One 11:e0145391CrossRefPubMedPubMedCentralGoogle Scholar
  74. 74.
    Jia LP, Qian Y, Zhang Y, Deng L, Liu LY, Zhu RN et al (2014) Prevalence and genetic diversity of noroviruses in outpatient pediatric clinics in Beijing, China 2010–2012. Infect Genet Evol 28:71–87CrossRefPubMedGoogle Scholar
  75. 75.
    Kim JS, Kim HS, Hyun J, Kim HS, Song W (2015) Molecular Epidemiology of Human Norovirus in Korea in 2013. Biomed Res Int 2015:468304PubMedPubMedCentralGoogle Scholar
  76. 76.
    Hoffmann D, Seebach J, Foley BT, Frosner G, Nadas K, Protzer U et al (2010) Isolated norovirus GII.7 strain within an extended GII.4 outbreak. J Med Virol 82:1058–1064CrossRefPubMedGoogle Scholar
  77. 77.
    Kwok K, Niendorf S, Lee N, Hung TN, Chan LY, Jacobsen S et al (2017) Increased detection of emergent recombinant norovirus GII.P16-GII.2 strains in young adults, Hong Kong, China, 2016–2017. Emerg Infect Dis 23:1852–1855CrossRefPubMedPubMedCentralGoogle Scholar
  78. 78.
    Vega E, andVinje J (2011) Novel GII.12 norovirus strain, United States, 2009–2010. Emerg Infect Dis 17:1516–1518PubMedPubMedCentralGoogle Scholar
  79. 79.
    Liu LT, Kuo TY, Wu CY, Liao WT, Hall AJ, Wu FT (2017) Recombinant GII.P16-GII.2 norovirus, Taiwan 2016. Emerg Infect Dis 23:1180–1183CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mahsa Farsi
    • 1
  • Fatemeh Roodbari
    • 1
  • Behrooz Nejati
    • 1
  • Arash Arashkia
    • 2
  • Somayeh Jalilvand
    • 3
  • Alireza Nateghian
    • 4
  • Aliakbar Rahbarimanesh
    • 5
  • Sayed Mahdi Marashi
    • 3
  • Zabihollah Shoja
    • 2
  1. 1.Biology Department, Faculty of Basic SciencesUniversity of MazandaranBabolsarIran
  2. 2.Virology DepartmentPasteur Institute of IranTehranIran
  3. 3.Virology Department, School of Public HealthTehran University of Medical SciencesTehranIran
  4. 4.Ali-Asghar Children’s HospitalIran University of Medical SciencesTehranIran
  5. 5.Department of Pediatric Infectious Diseases, Bahrami Children HospitalTehran University of Medical SciencesTehranIran

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