Abstract
Tetherin is an anti-viral factor that restricts viral budding through tethering virions to the cell surface. The human parainfluenza virus type 2 (hPIV-2) V protein decreases cell surface tetherin in HeLa cells, which constitutively express endogenous tetherin. However, the role of the hPIV-2 V protein in tetherin induction remains unclear. Here, we examined whether hPIV-2 infection itself induces tetherin in HEK293 cells that have no basal expression of tetherin. Unlike influenza A virus (IAV) infection, hPIV-2 infection induced neither tetherin mRNA nor protein expression. In contrast, robust tetherin induction was observed by infection of rPIV-2s carrying V mutants, in which either three Trp residues (W178H/W182E/W192A) or Cys residues (C209/211/214A) that are important for decreasing cell surface tetherin are mutated. hPIV-2 infection also inhibited the induction of tetherin expression by IFN-α and IAV infection. Furthermore, hPIV-2 V protein but not P and VW178H/W182E/W192A suppressed tetherin induction. Our data collectively suggest that the hPIV-2 V protein inhibits tetherin expression induced by several external stimuli.
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Acknowledgements
We thank Drs. Klaus Strebel and Amy Andrew for anti-Bst2 via the NIH AIDS Research and Reference Reagent Program.
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Ohta, K., Matsumoto, Y., Yumine, N. et al. Human parainfluenza virus type 2 V protein inhibits induction of tetherin. Med Microbiol Immunol 206, 311–318 (2017). https://doi.org/10.1007/s00430-017-0508-z
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DOI: https://doi.org/10.1007/s00430-017-0508-z