Screening and identification of host factors interacting with UL14 of herpes simplex virus 1
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The UL14 protein of herpes simplex virus type 1 (HSV-1) is highly conserved in herpesvirus family. However, its exact function during the HSV-1 replication cycle is little known. In the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with UL14, and five target candidates were yielded: (1) TSC22 domain family protein 3 (TSC22D3); (2) Mediator of RNA polymerase II transcription subunit 8 isoform 1(MED8); (3) Runt-related transcription factor 3 (RUNX3); (4) Arrestin beta-2 (ARRB2); (5) Cereblon (CRBN). Indirect immunofluorescent assay showed that both TSC22D3 and MED8 co-localized with UL14. Co-immunoprecipitation assay demonstrated that UL14 could be immunoprecipitated by TSC22D3, suggesting that UL14 interacted with TSC22D3 under physiological condition. In summary, this study opened up new avenues toward delineating the function and physiological significance of UL14 during the HSV-1 replication cycle.
KeywordsHerpes simplex virus 1 UL14 Yeast two-hybrid Co-localization Co-immunoprecipitation
This work was supported by grants from the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences (20071010-141); the National Natural Science Foundation of China (30870120, 30900059 and 81000736); the Major State Basic Research Development Program of China (2010CB530105 and 2011CB504802). We thank Dr. Pallardy for the generous gift plasmid TSC22D3-Myc.
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