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Influence of Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxododecanoyl) homoserine lactone on mast cells

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Abstract

Quorum sensing system is a cell-to-cell communication system that plays a pivotal role in virulence expression in bacteria. Recent advances have demonstrated that the Pseudomonas aeruginosa quorum sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC12-HSL), exerts effects on mammalian cells and modulates host immune response. Mast cells (MCs) are strategically located in the tissues that are constantly exposed to external stimulus. Therefore, it is very much possible that 3OC12-HSL may interact with MCs. Little is known, however, about specific effects of 3OC12-HSL on MCs. To address this, we investigated the influence of 3OC12-HSL on cell viability, apoptosis, intracellular calcium and cytokine release in MCs. We found that at high concentrations (100 μM), 3OC12-HSL inhibited proliferation and induced apoptosis in P815. The 3OC12-HSL treatment significantly increased intracellular calcium release in both P815 and HMC-1. We also observed that 3OC12-HSL-induced histamine release and degranulation in HMC-1 cells. Furthermore, 3OC12-HSL-induced IL-6 production at lower concentrations (6.25–12.5 μM) but steadily reduced IL-6 production at high concentration (50–100 μM). These data demonstrate that P. aeruginosa 3OC12-HSL affects MCs function.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (30571647), China National 973 project (MOST, 2007CB512900). We thank Professor Zhong, RQ (Changzheng Hospital, Shanghai, China) for kindly providing human mast cell line HMC-1.

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  1. Department of Infectious Diseases, Tongji Hospital, Wuhan, China

    Hongtao Li, Lili Wang, Lu Ye, Yan Mao, Xuhua Xie, Chao Xia, Jia Chen & Jianxin Song

  2. Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Zimin Lu

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  1. Hongtao Li
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Li, H., Wang, L., Ye, L. et al. Influence of Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxododecanoyl) homoserine lactone on mast cells. Med Microbiol Immunol 198, 113–121 (2009). https://doi.org/10.1007/s00430-009-0111-z

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