Medical Microbiology and Immunology

, Volume 196, Issue 3, pp 135–143 | Cite as

PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells

  • Yanming Zou
  • Lina He
  • Chun-Hua Wu
  • Hong Cao
  • Zhi-Hua Xie
  • Yannan Ouyang
  • Yang Wang
  • Ambrose Jong
  • Sheng-He HuangEmail author
Original Investigation


During the development of Escherichia coli K1 meningitis, interaction between E. coli invasion protein IbeA and surface protein(s) on human brain microvascular endothelial cells (HBMEC) is required for invasion and IbeA-mediated signaling. Here, an IbeA-binding protein was identified as polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF). The specific binding was confirmed by ligand overlay assay. The cell surface-expressed PSF was verified by the confocal microscopy. Recombinant PSF blocked E. coli K1 invasion of HBMEC effectively. Overexpression of PSF in the lentivirus-transducted HBMEC significantly enhanced E. coli K1 invasion. These results suggest that IbeA interacts with PSF for the E. coli K1 invasion of HBMEC.


IbeA Meningitis PSF Brain microvascular endothelial cells E. coli K1 



We thank Dr. Kwang S. Kim for providing brain endothelial cells, Dr. Yu-Zhou Yang for anti-His tag monoclonal antibodies, Dr. Wei Li for the lentivirus transduction system and Dr. James G. Patton for PSF constructs and antibodies. This project is financially supported by Public Health Service grants R01-AI40635 (S.H.H.) and R01-NS047599 (A.J.).


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yanming Zou
    • 1
  • Lina He
    • 1
  • Chun-Hua Wu
    • 1
  • Hong Cao
    • 2
  • Zhi-Hua Xie
    • 3
  • Yannan Ouyang
    • 1
  • Yang Wang
    • 1
  • Ambrose Jong
    • 1
  • Sheng-He Huang
    • 1
    • 2
    • 4
    Email author
  1. 1.Saban Research InstituteChildrens Hospital Los AngelesLos AngelesUSA
  2. 2.Department of MicrobiologySouthern Medical UniversityGuangzhouChina
  3. 3.ProteinX LabSan DiegoUSA
  4. 4.Department of Pediatrics, Childrens Hospital Los AngelesUniversity of Southern CaliforniaLos AngelesUSA

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