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Rapid detection of herpes simplex virus DNA in cerebrospinal fluid: comparison between loop-mediated isothermal amplification and real-time PCR

Medical Microbiology and Immunology volume 194, pages 181–185 (2005)Cite this article

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.

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Acknowledgements

We thank the following people for their contributions to this study: Dr. Masahide Futamura (Aichi Prefectural Colony Hospital), Dr. Hisanori Sobajima (Nagoya City University School of Medicine), Dr. Masahiro Hayakawa (Nagoya University Hospital), Drs. Eiko Kato and Chizuko Suzuki (Nagoya First Red Cross Hospital), Dr. Makoto Ohshiro (Ogaki Municipal Hospital), Dr Youhei Tamura (the Jikei University School of Medicine), Dr. Toshiko Kubota (Kakegawa City General Hospital), Dr. Chie Nakamura (Higashi Municipal Hospital of Nagoya), Drs. Fumi Ishikawa and Toshiyuki Suzuki (Tsuchiura Kyodo General Hospital). This work was supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology (16591022).

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Affiliations

  1. Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya, 466-8550, Japan

    Hiroshi Kimura, Jun-ichi Kawada & Yoshinori Ito

  2. Department of Medical Information Technology, Fujita Health University College, Toyoake, Japan

    Masaru Ihira

  3. Department of Pediatrics, Fujita Health University College, Toyoake, Japan

    Yoshihiro Enomoto, Tetsushi Yoshikawa & Yoshizo Asano

  4. Department of Pediatrics, Graduate school of Medicine and Dentistry, Okayama University, Okayama, Japan

    Tsuneo Morishima

Authors
  1. Hiroshi Kimura
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  2. Masaru Ihira
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  3. Yoshihiro Enomoto
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  4. Jun-ichi Kawada
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  5. Yoshinori Ito
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  6. Tsuneo Morishima
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  7. Tetsushi Yoshikawa
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  8. Yoshizo Asano
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Correspondence to Hiroshi Kimura.

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Kimura, H., Ihira, M., Enomoto, Y. et al. Rapid detection of herpes simplex virus DNA in cerebrospinal fluid: comparison between loop-mediated isothermal amplification and real-time PCR. Med Microbiol Immunol 194, 181–185 (2005). https://doi.org/10.1007/s00430-005-0242-9

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  • DOI: https://doi.org/10.1007/s00430-005-0242-9

Keywords

  • HSV
  • LAMP
  • Real-time PCR
  • CSF
  • Encephalitis