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Cellular receptor for pixuna virus in chicken embryonic fibroblasts

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Abstract

In this study, we describe the isolation and partial characterization of a Pixuna virus receptor, which is a component of a plasma membrane fraction of chicken embryo fibroblast (CEF). Polyclonal antiserum was prepared from rabbits immunized with the membrane fraction. Said polyclonal antiserum reacted in a similar way as monoclonal antibodies raised against the membrane fraction. Both antisera were able to prevent CEF and Vero cells from infection with Pixuna virus. Immunofluorescence studies suggested that the receptors found in the fibroblasts and in the Vero cells shared at least some epitopes. The Western blot analysis of the purified membrane fraction antigens, which reacted with the monoclonal and polyclonal antibodies, detected a double band with a molecular mass of approximately 60 kDa. Not only immunofluorescence staining but also electron and immunoelectron microscopy studies evidenced the receptor localization in the plasma membrane. In this manner, we reported the isolation and partial characterization of a new Pixuna virus receptor in the plasma membrane of chicken embryo fibroblasts in culture. The data obtained demonstrated the receptor significance for the penetration of Pixuna virus into fibroblasts and mammalian cell and the related importance of designing new antiviral drugs by blocking the mechanism of receptor penetration of the virus into the cells.

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Acknowledgements

GP is a member of the Research Career from the Consejo Nacional de Investigaciones Científicas y Técnicas de la R. Argentina and contributed principally to this work. We gratefully acknowledge to Sondra Schlesinger and Marta Ruben-Campioni for fruitful and excellent critiques of the manuscript. We appreciate the assessment and language editing work carried out by Valeria Mosqueda and Lorena Riquelme. We thank Avicola Bartolucci that provided free the chicken embryo and the 1-day-old chicken. We also thank Cristina Maldonado for assistance with electron microscopy of the receptor as well as to Maria del Pilar Díaz for the statistical data and to Brenda Königheim, Javier Aguilar and Luciana Martinez from our Institute for the supply of Vero cells in the final stage of this study. Part of this work was supported from SECyT, UNC.

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  1. Laboratorio de Receptores Celulares, Instituto de Virología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina

    G. Paglini, E. del C. Pereyra & S. Paglini

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  1. G. Paglini
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  2. E. del C. Pereyra
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  3. S. Paglini
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Correspondence to S. Paglini.

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Paglini, G., Pereyra, E.d.C. & Paglini, S. Cellular receptor for pixuna virus in chicken embryonic fibroblasts. Med Microbiol Immunol 195, 85–92 (2006). https://doi.org/10.1007/s00430-005-0004-8

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  • DOI: https://doi.org/10.1007/s00430-005-0004-8

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