The development of the pressure-bearing tendon of the bullfrog, Rana catesbeiana

Abstract

 The plantaris longus tendon of the bullfrog is a pressure-bearing tendon and develops a fibrocartilage-like arrangement in the area subjected to compressive forces. The fibrocartilage-like tissue shows some distinct aspects of cellular and fibrillar structure and distribution as compared to the mammalian counterparts. In this work, the development of the plantaris longus tendon was assessed by investigating some of its structural, cytochemical and immunocytochemical aspects in developing tadpoles. The pressure-bearing region is structurally distinct from the tension region as early as at stage 35 of larval development. There is little extracellular matrix in both regions, but the former shows round mesenchymal-like cells with many processes and cell junctions, while the latter is populated by fibroblasts. As development proceeds, the cells in the compression region retract the processes, loose the connections to each other, become rounded and produce abundant proteoglycans and some collagen fibers. Progressively, their organelles become localized in a restricted perinuclear area and are surrounded by a constantly increasing amount of vimentin. The fibroblasts of the tension region produce mostly collagen fibrils, which are packed and aligned to each other. These cells become more elongated and show a diminished cytoplasmic area. The results allow for the conclusion that the compression region does not arise by simple hyperplasia of the peripheral layers of a normal tendon, but from a programmed sequence of developmental steps. This assumption is based on the fact that muscle fibers are still developing when the tendon is already showing a differentiated compression region. We further suggest that mechanical stimulation is a secondary factor most likely associated with the maintenance of the differentiated phenotype of this tendon.