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Brain Structure and Function

, Volume 223, Issue 8, pp 3633–3652 | Cite as

Cortical networks of the mouse brain elaborate within the gray matter

  • Akiya Watakabe
  • Junya Hirokawa
Original Article
  • 268 Downloads

Abstract

In primates, proximal cortical areas are interconnected via within-cortex “intrinsic” pathway, whereas distant areas are connected via “extrinsic” white matter pathway. To date, such distinction has not been clearly done for small-brained mammals like rodents. In this study, we systematically analyzed the data of Allen Mouse Brain Connectivity Atlas to answer this question and found that the ipsilateral cortical connections in mice are almost exclusively contained within the gray matter, although we observed exceptions for projections from the retrosplenial area and the medial/orbital frontal areas. By analyzing axonal projections within the gray matter using Cortical Box method, which enabled us to investigate the layer patterns across different cortical areas, we obtained the following results. First, widespread axonal projections were observed in both upper and lower layers in the vicinity of injections, whereas highly specific “point-to-point” projections were observed toward remote areas. Second, such long-range projections were predominantly aligned in the anteromedial–posterolateral direction. Third, in the majority of these projections, the connecting axons traveled through layer 6. Finally, the projections from the primary and higher order areas to distant targets preferentially terminated in the middle and superficial layers, respectively, suggesting hierarchical connections similar to those of primates. Overall, our study demonstrated conspicuous differences in gray/white matter segregation of axonal projections between rodents and primates, despite certain similarities in the hierarchical cortical organization.

Keywords

AAV Anterograde tracers Cortico-cortical projection Feedforward Feedback White matter 

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Riken, Center for Brain ScienceWakoJapan
  2. 2.Laboratory of Neural InformationDoshisha UniversityKyotanabeJapan

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