Brain Structure and Function

, Volume 223, Issue 3, pp 1121–1132 | Cite as

High thickness histological sections as alternative to study the three-dimensional microscopic human sub-cortical neuroanatomy

  • Eduardo Joaquim Lopes Alho
  • Ana Tereza Di Lorenzo Alho
  • Lea Grinberg
  • Edson AmaroJr.
  • Gláucia Aparecida Bento dos Santos
  • Rafael Emídio da Silva
  • Ricardo Caires Neves
  • Maryana Alegro
  • Daniel Boari Coelho
  • Manoel Jacobsen Teixeira
  • Erich Talamoni Fonoff
  • Helmut Heinsen
Original Article

Abstract

Stereotaxy is based on the precise image-guided spatial localization of targets within the human brain. Even with the recent advances in MRI technology, histological examination renders different (and complementary) information of the nervous tissue. Although several maps have been selected as a basis for correlating imaging results with the anatomical locations of sub-cortical structures, technical limitations interfere in a point-to-point correlation between imaging and anatomy due to the lack of precise correction for post-mortem tissue deformations caused by tissue fixation and processing. We present an alternative method to parcellate human brain cytoarchitectural regions, minimizing deformations caused by post-mortem and tissue-processing artifacts and enhancing segmentation by means of modified high thickness histological techniques and registration with MRI of the same specimen and into MNI space (ICBM152). A three-dimensional (3D) histological atlas of the human thalamus, basal ganglia, and basal forebrain cholinergic system is displayed. Structure’s segmentations were performed in high-resolution dark-field and light-field microscopy. Bidimensional non-linear registration of the histological slices was followed by 3D registration with in situ MRI of the same subject. Manual and automated registration procedures were adopted and compared. To evaluate the quality of the registration procedures, Dice similarity coefficient and normalized weighted spectral distance were calculated and the results indicate good overlap between registered volumes and a small shape difference between them in both manual and automated registration methods. High thickness high-resolution histological slices in combination with registration to in situ MRI of the same subject provide an effective alternative method to study nuclear boundaries in the human brain, enhancing segmentation and demanding less resources and time for tissue processing than traditional methods.

Keywords

Cytoarchitecture Thalamus Sub-cortical atlas Magnetic resonance imaging 

Notes

Acknowledgements

The authors would like to thank the team participating on the São Paulo-Würzburg collaborative project. This includes all members of the Brain Bank of the Brazilian Aging Brain Research Group (BBBABSG) of the University of São Paulo Medical School, Mrs. E. Broschk and Mrs. A. Bahrke from the Morphological Brain Research Unit of the University of Würzburg, Germany.

Compliance with ethical standards

Funding source

This study was supported by resources from the University of Sao Paulo School of Medicine, Brazil and University of Würzburg, Germany. The author Eduardo Joaquim Lopes Alho was supported by a scholarship from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) agency, Brazil, for doctoral studies at the University of Würzburg, Germany. The authors do not have personal financial or institutional interest in any of the drugs, materials, or devices described in this article.

Conflict of interest

The authors disclose any actual or potential conflict of interest including any financial, personal, or other relationships with other people or organizations within 3 years of beginning the submitted work that could inappropriately influence, or be perceived to influence, their work.

Ethical standards

The work described has been carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki).

Supplementary material

Supplementary material 1 (WMV 26324 kb)

Supplementary material 2 (WMV 21876 kb)

Supplementary material 3 (WMV 188761 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Eduardo Joaquim Lopes Alho
    • 1
    • 2
    • 4
    • 7
  • Ana Tereza Di Lorenzo Alho
    • 3
    • 4
  • Lea Grinberg
    • 3
    • 5
  • Edson AmaroJr.
    • 4
  • Gláucia Aparecida Bento dos Santos
    • 3
    • 4
  • Rafael Emídio da Silva
    • 4
  • Ricardo Caires Neves
    • 3
  • Maryana Alegro
    • 4
    • 5
  • Daniel Boari Coelho
    • 6
  • Manoel Jacobsen Teixeira
    • 2
  • Erich Talamoni Fonoff
    • 2
  • Helmut Heinsen
    • 1
    • 4
  1. 1.Morphological Brain Research Unit, Department of PsychiatryUniversity of WürzburgWürzburgGermany
  2. 2.Division of Functional Neurosurgery, Department of NeurologyUniversity of São Paulo Medical SchoolSão PauloBrazil
  3. 3.Department of PathologyUniversity of São Paulo Medical SchoolSão PauloBrazil
  4. 4.Department of RadiologyUniversity of São Paulo Medical SchoolSão PauloBrazil
  5. 5.Sandler Neurosciences Center, Memory and Aging Center, Department of NeurologyUniversity of California at San FranciscoSan FranciscoUSA
  6. 6.Human Motor Systems Laboratory, School of Physical Education and SportUniversity of São PauloSão PauloBrazil
  7. 7.São PauloBrazil

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