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Brain Structure and Function

, Volume 223, Issue 3, pp 1133–1148 | Cite as

Perineuronal nets labeled by monoclonal antibody VC1.1 ensheath interneurons expressing parvalbumin and calbindin in the rat amygdala

  • Alexander J. McDonald
  • Patricia G. Hamilton
  • Colin J. Barnstable
Original Article

Abstract

Perineuronal nets (PNNs) are specialized condensations of extracellular matrix that ensheath particular neuronal subpopulations in the brain and spinal cord. PNNs regulate synaptic plasticity, including the encoding of fear memories by the amygdala. The present immunohistochemical investigation studied PNN structure and distribution, as well as the neurochemistry of their ensheathed neurons, in the rat amygdala using monoclonal antibody VC1.1, which recognizes a glucuronic acid 3-sulfate glycan associated with PNNs in the cerebral cortex. VC1.1+ PNNs surrounded the cell bodies and dendrites of a subset of nonpyramidal neurons in cortex-like portions of the amygdala (basolateral amygdalar complex, cortical nuclei, nucleus of the lateral olfactory tract, and amygdalohippocampal region). There was also significant neuropilar VC1.1 immunoreactivity, whose density varied in different amygdalar nuclei. Cell counts in the basolateral nucleus revealed that virtually all neurons ensheathed by VC1.1+ PNNs were parvalbumin-positive (PV+) interneurons, and these VC1.1+/PV+ cells constituted 60% of all PV+ interneurons, including all of the larger PV+ neurons. Approximately 70% of VC1.1+ neurons were calbindin-positive (CB+), and these VC1.1+/CB+ cells constituted about 40% of all CB+ neurons. Colocalization of VC1.1 with Vicia villosa agglutinin (VVA) binding, which stains terminal N-acetylgalactosamines, revealed that VC1.1+ PNNs were largely a subset of VVA+ PNNs. This investigation provides baseline data regarding PNNs in the rat which should be useful for future studies of their function in this species.

Keywords

Extracellular matrix Perineuronal nets Amygdala Calcium-binding proteins Immunohistochemistry 

Notes

Acknowledgements

The authors thank Dr. Kenneth G. Baimbridge (University of British Columbia) for his generous donation of the PV and CB polyclonal antisera. This work was supported by NIH Grants R01MH104638 and R01NS19733.

Compliance with ethical standards

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Human and animal rights statement

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Alexander J. McDonald
    • 1
  • Patricia G. Hamilton
    • 1
  • Colin J. Barnstable
    • 2
  1. 1.Department of Pharmacology, Physiology and NeuroscienceUniversity of South Carolina School of MedicineColumbiaUSA
  2. 2.Department of Neural and Behavioral SciencesThe Pennsylvania State University College of MedicineHersheyUSA

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