Abstract
Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli–Seip congenital lipodystrophy (Bscl2) gene mutations.
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Acknowledgments
This work was supported by the Australian Research Council Centre of Excellence for Integrative Brain Function (ARC Centre Grant CE140100007) and a project grant (1027387) from the National Health and Medical Research Council of Australia. H. Yang is a Senior Research Fellow of the NHMRC. We appreciate Dr Emma Schofield’s help in proof reading this manuscript.
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All procedures were in compliance with the ethical standards of the Animal Care and Ethics Committee of The University of New South Wales.
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Liu, X., Xie, B., Qi, Y. et al. The expression of SEIPIN in the mouse central nervous system. Brain Struct Funct 221, 4111–4127 (2016). https://doi.org/10.1007/s00429-015-1151-3
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DOI: https://doi.org/10.1007/s00429-015-1151-3