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Purkinje cell compartmentalization in the cerebellum of the spontaneous mutant mouse dreher

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Abstract

The cerebellar morphological phenotype of the spontaneous neurological mutant mouse dreher (Lmx1a dr-J) results from cell fate changes in dorsal midline patterning involving the roof plate and rhombic lip. Positional cloning revealed that the gene Lmx1a, which encodes a LIM homeodomain protein, is mutated in dreher, and is expressed in the developing roof plate and rhombic lip. Loss of Lmx1a causes reduction of the roof plate, an important embryonic signaling center, and abnormal cell fate specification within the embryonic cerebellar rhombic lip. In adult animals, these defects result in variable, medial fusion of the cerebellar vermis and posterior cerebellar vermis hypoplasia. It is unknown whether deleting Lmx1a results in displacement or loss of specific lobules in the vermis. To distinguish between an ectopic and absent vermis, the expression patterns of two Purkinje cell-specific compartmentation antigens, zebrin II/aldolase C and the small heat shock protein HSP25 were analyzed in dreher cerebella. The data reveal that despite the reduction in volume and abnormal foliation of the cerebellum, the transverse zones and parasagittal stripe arrays characteristic of the normal vermis are present in dreher, but may be highly distorted. In dreher mutants with a severe phenotype, zebrin II stripes are fragmented and distributed non-symmetrically about the cerebellar midline. We conclude that although Purkinje cell agenesis or selective Purkinje cell death may contribute to the dreher phenotype, our data suggest that aberrant anlage patterning and granule cell development lead to Purkinje cell ectopia, which ultimately causes abnormal cerebellar architecture in dreher.

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Acknowledgments

These studies were supported by grants from the Canadian Institutes of Health Research (RH), the CIHR Training Program in Genetics, Child Development and Health (RVS), and NIH R01 NS050386 (KJM). RVS is currently supported by new investigator start-up funds from Baylor College of Medicine and Texas Children’s Hospital, by the Caroline Wiess Law Fund for Research in Molecular Medicine, a BCM IDDRC Project Development Award, and by a BCM IDDRC Grant (Number 5P30HD024064) from the Eunice Kennedy Shriver National Institute Of Child Health and Human Development.

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  1. Department of Pathology and Immunology, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute of Texas Children’s Hospital, 1250 Moursund Street, Suite 1325, Houston, TX, 77030, USA

    Roy V. Sillitoe & Nicholas A. George-Jones

  2. Department of Neuroscience, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute of Texas Children’s Hospital, 1250 Moursund Street, Suite 1325, Houston, TX, 77030, USA

    Roy V. Sillitoe & Nicholas A. George-Jones

  3. Department of Pediatrics, Seattle Children’s Hospital Research Institute Center for Integrative Brain Research, The University of Washington, 1900 Ninth Avenue C9S-10, Seattle, WA, 98101, USA

    Kathleen J. Millen

  4. Department of Cell Biology and Anatomy, Faculty of Medicine, Hotchkiss Brain Institute, The University of Calgary, Calgary, AB, T2N 4N1, Canada

    Richard Hawkes

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  1. Roy V. Sillitoe
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Sillitoe, R.V., George-Jones, N.A., Millen, K.J. et al. Purkinje cell compartmentalization in the cerebellum of the spontaneous mutant mouse dreher . Brain Struct Funct 219, 35–47 (2014). https://doi.org/10.1007/s00429-012-0482-6

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