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Hippocampal glutamate transporter 1 (GLT-1) complex levels are paralleling memory training in the Multiple T-Maze in C57BL/6J mice

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Abstract

The glutamate transporter 1 (GLT-1) is essential for glutamate uptake in the brain and associated with various psychiatric and neurological disorders. Pharmacological inhibition of GLT-1 results in memory deficits, but no study linking native GLT-1 complexes was published so far. It was therefore the aim of the study to associate this highly hydrophobic, eight transmembrane spanning domains containing transporter to memory training in the Multiple T-maze (MTM). C57BL/6J mice were used for the spatial memory training experiments, and trained mice were compared to untrained (yoked) animals. Mouse hippocampi were dissected out 6 h after training on day 4, and a total enriched membrane fraction was prepared by ultracentrifugation. Membrane proteins were separated by blue native polyacrylamide gel electrophoresis (BN-PAGE) with subsequent Western blotting against GLT-1 on these native gels. Moreover, GLT-1 complexes were identified by mass spectrometry (nano-LC-ESI-MS/MS). Animals learned the MTM task and multiple GLT-1 complexes were detected at apparent molecular weights of 242, 480 and 720 kDa on BN-PAGE Western blotting. GLT-1 complex levels were significantly higher in the trained group as compared to yoked controls, and antibody specificity was verified by immunoblotting on multidimensional gels. Hippocampal GLT-1 was unambiguously identified by mass spectrometry with high sequence coverage, and glycosylation was observed. It is revealed that increased GLT-1 complex levels are paralleling and are linked to spatial memory training. We provide evidence that signal termination, represented by the excitatory amino acid transporter GLT-1 complexes, is involved in spatial memory mechanisms.

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Acknowledgments

The authors acknowledge the contribution by the Verein zur Durchführung der wissenschaftlichen Forschung auf dem Gebiet der Neonatologie und Kinderintensivmedizin “Unser Kind”.

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  1. Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Währinger Gürtel 18, 1090, Vienna, Austria

    Seok Heo, Gangsoo Jung, Tamara Beuk & Gert Lubec

  2. Core Unit of Biomedical Research, Division of Laboratory Animal Science and Genetics, Medical University of Vienna, Brauhausgasse 34, 2325, Himberg, Austria

    Harald Höger

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  1. Seok Heo
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Correspondence to Gert Lubec.

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Seok Heo and Gangsoo Jung contributed equally to this work.

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Heo, S., Jung, G., Beuk, T. et al. Hippocampal glutamate transporter 1 (GLT-1) complex levels are paralleling memory training in the Multiple T-Maze in C57BL/6J mice. Brain Struct Funct 217, 363–378 (2012). https://doi.org/10.1007/s00429-011-0362-5

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