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Three-dimensional reconstructions from non-deparaffinized tissue sections

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Abstract

Three-dimensional (3-D) reconstruction from microscopic images represents a useful tool for the study of biological structures in embryology and developmental biology. However, it is usually necessary to cope with many difficulties connected with the preparation of specimens. In order to minimize mutual displacement of structures in successive sections, the applicability of non-deparaffinized tissue sections for 3-D reconstruction was tested. Chicken embryos were fixed and stained in toto with eosin and then embedded in paraffin. About 30-μm-thick non-deparaffinized serial sections were used for obtaining initial data for 3-D reconstruction of larger stacks of embryonic bodies using either fluorescence or confocal microscope. The same sections served for both collecting optical serial sections of mesonephros as source images for its 3-D reconstruction, and immunohistochemical detection of fibronectin, laminin and vimentin. It was found that sections with retained paraffin preserve the mutual spatial relationships of tissue components as well as provide an excellent differentiation of structure. It makes the process of 3-D reconstruction easier. The localization of the products of immunohistochemical reactions demonstrated the co-localization of fibronectin and laminin in basal laminas and the presence of vimentin in glomeruli and mesenchymal tissue. The use of non-deparaffinized sections represents a less time consuming and more effective alternative to thin histological sections for the purpose of 3-D reconstruction, and enables further application of material.

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References

  • Åslund N, Carlsson K, Liljeborg A, Majlof L (1983) PHOIBOS, a microscope scanner designed for micro-fluorometric applications, using laser induced fluorescence. In: Proceedings of the third Scandinavian conference on image analysis, Studentliteratur, Lund, p 338

  • Apgar JM, Juarranz A, Espada J, Villanueva A, Caňete M, Stockert JC (1998) Fluorescence microscopy of rat embryo sections stained with haematoxylin-eosin and Masson’s trichrome method. J Microsc 191:20–27

    Article  PubMed  CAS  Google Scholar 

  • Arnold WH, Lang T (2001) Development of the membranous labyrinth of human embryos and fetuses using computer aided 3D- reconstruction. Ann Anat 183:61–66

    PubMed  CAS  Google Scholar 

  • Arnold WH, Lang M, Sperber GH (2001) 3D- reconstruction of craniofacial structures of a human anencephalic fetus. Case report. Ann Anat 183:67–71

    Article  PubMed  CAS  Google Scholar 

  • Baba N (1992) Computer aided three-dimensional reconstruction from serial section images. In: Häder DP (ed) Image analysis in biology. CRC Press, Boca Raton, pp 251–270

    Google Scholar 

  • Beck JC, Murray JA, Willows AO, Cooper MS (2000) Computer-assisted visualizations of neural networks: expanding the field of view using seamless confocal montaging. J Neurosc Methods 98:155–163

    Article  CAS  Google Scholar 

  • Brakenhoff GJ, van der Voort HTM, van Spronsen EA, Linnemans WAM, Nanninga N (1985) Three dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy. Nature 317:748–749

    Article  PubMed  CAS  Google Scholar 

  • Bron C, Gremillet P (1992) 3-D reconstructions by image-processing of serial sections in electron microscopy. In: Kriete A (ed) Visualization in biomedical microscopies. 3-D Imaging and computer applications. VCH, Weinheim, pp 75–105

    Google Scholar 

  • Carlsson K, Danielsson P, Lenz R, Liljeborg A, Majlof L, Å slund N (1985) Three-dimensional microscopy using a confocal laser scanning microscope. Opt Lett 10:53–55

    Google Scholar 

  • Carretero A, Ditrich H, Pérez-Aparicio FJ, Splechtna H, Ruberte J (1995) Development and degeneration of the arterial system in the mesonephros and metanephros of chicken embryo. Anat Rec 243:120–128

    Article  PubMed  CAS  Google Scholar 

  • Carretero A, Ditrich H, Navarro M, Ruberte J (1997) Afferent portal venous system in the mesonephros and metanephros of chick embryos: development and degeneration. Anat Rec 247:63–70

    Article  PubMed  CAS  Google Scholar 

  • Clarke GE, Hamilton PW, Montgomery WA (1993) Aligning histological serial sections for three-dimensional reconstruction using an excimer laser beam. Pathol Res Pract 189:563–566

    PubMed  CAS  Google Scholar 

  • Deverell MH, Whimster WF (1989) A method of image registration for three-dimensional reconstruction of microscopic structures using an IBAS 2000 image analysis system. Pathol Res Pract 185:602–605

    PubMed  CAS  Google Scholar 

  • Ditrich H, Lametschwandtner A (1992) Glomerular development and growth of the renal blood vascular system in Xenopus laevis (Amphibia: Anura: Pipidae) during metamorphic climax. J Morphol 213:335–340

    Article  PubMed  CAS  Google Scholar 

  • Ditrich H, Splechtna H (1990) Kidney structure investigations using scanning electron microscopy of corrosion casts—a state of art review. Scan Microsc 4:943–956

    CAS  Google Scholar 

  • Fraser AM, Swinney HL (1986) Independent coordinates for strange attractor from mutual information. Phys Rev A 33:1134–1140

    Article  PubMed  Google Scholar 

  • Friebová Z (1975) Formation of the chick mesonephros. 1. General outline of development. Fol Morphol (Prague) 23:19–28

    Google Scholar 

  • Friebová-Zemanová (1981) Formation of the chick mesonephros. 4. Course and architecture of the developing nephrons. Anat Embryol 161:341–354

    Article  PubMed  Google Scholar 

  • Friebová-Zemanová Y, Goncharevskaya OA (1982) Foramtion of the chick mesonephros. 5. Spatial distribution of the nephron populations. Anat Embryol 165:125–139

    Article  PubMed  Google Scholar 

  • Fujimura A, Nozaka Y (2002) Analysis of the three-dimensional lymphatic architecture of the periodontal tissue using a new 3D reconstruction method. Microsc Res Tech 56:60–65

    Article  PubMed  Google Scholar 

  • Glaser EM, Van Der Loos H (1965) A semi-automatic computer microscope for the analysis of neuronal morphology. IEEE Trans Biomed Eng 2:22–31

    Google Scholar 

  • Guo M, Ditrich H, Splechtna H (1996) Renal vascular and uriniferous tubules in the common iguana. J Morphol 229:97–104

    Article  Google Scholar 

  • Hell S, Reiner G, Cremer C, Stelzer EHK (1993) Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J Microsc 169:391–405

    Google Scholar 

  • Hibbard LS, Grothe RA, Arnicar-Sulze TL, Dovey-Hartman BJ, Page RB (1993) Computed three-dimensional reconstruction of median-eminence capillary modules: image alignment and correlation. J Microsc 171:39–56

    PubMed  CAS  Google Scholar 

  • Hiruma T, Nakamura H (2003) Origin and development of the pronephros in the chick embryo. J Anat 203:539–552

    Article  PubMed  Google Scholar 

  • Huijsmans DP, Lamers WH, Los JA, Strackee J (1986) Toward computerized morphometric facilities: a review of 58 software packages for computer-aided three-dimensional reconstruction, quantification, and picture generation from parallel serial sections. Anat Rec 216:449–470

    Article  PubMed  CAS  Google Scholar 

  • Janáček J, Kubínová L (1998) 3D reconstruction of surfaces captured by confocal microscopy. Acta Stereol 17:259–264

    Google Scholar 

  • Jirkovská M, Kubínová L, Krekule I, Karen P, Palovský R (1994) To the applicability of common fluorescent dyes in confocal microscopy. Funct Develop Morphol 4:171–172

    Google Scholar 

  • Jirkovská M, Kubínová L, Krekule I, Hach P (1998) Spatial arrangement of fetal placental capillaries in terminal villi: a study using confocal microscopy. Anat Embryol 197:263–272

    Article  PubMed  Google Scholar 

  • Jirkovská M, Kubínová L, Janáček J, Moravcová M, Krejčí V, Karen P (2002) Topological properties and spatial organization of villous capillaries in normal and diabetic placentas. J Vasc Res 39(3):268–278

    Article  PubMed  Google Scholar 

  • Jirsová Z, Zemanová Z, Jirkovská M (2003) Development of glomerular and peritubular vascular bed of the chick mesonephros. Plzeň Lék Sborn 78:111–113

    Google Scholar 

  • Karen P, Jirkovská M, Tomori Z, Demjénová E, Janáček J, Kubínová L (2003) Three dimensional computer reconstruction of large tissue volumes based on composing series of high-resolution confocal images by GlueMRC and LinkMRC software. Microsc Res Tech 62:415–422

    Article  PubMed  Google Scholar 

  • Kaufman MH, Kaufman DB, Brune RM, Stark M, Armstrong JF, Clarke AR (1997) Analysis of fused maxillary incisor dentition in p53-deficient exencephalic mice. J Anat 191:57–64

    Article  PubMed  Google Scholar 

  • Kay PA, Robb RA, Bostwick DG (1998) Prostate cancer microvessels: a novel method for three-dimensional reconstruction and analysis. Prostate 37:270–277

    Article  PubMed  CAS  Google Scholar 

  • Knabe W, Washausen S, Brunnett G, Kuhn HJ (2002) Use of ’reference series’ to realign histological serial sections for three-dimensional reconstructions of the positions of cellular events in the developing brain. J Neurosc Meth 121:169–180

    Article  Google Scholar 

  • Kriete A (eds) (1992) Visualization in biomedical microscopies. 3-D Imaging and computer applications. Weinheim VCH, New York

  • Kriete A, Magdowski G (1990) Computerized three-dimensional reconstructions of serial sections in electron microscopy. Ultramicroscopy 32:48–54

    Article  PubMed  CAS  Google Scholar 

  • Kriete A, Schwebel T (1996) 3-D TOP-A software package for the topological analysis of image sequences. J Struct Biol 116:150–154

    Article  PubMed  CAS  Google Scholar 

  • Kropf N, Sobel I, Levinthal C (1985) Serial section reconstruction using CARTOS. In: Mize RR (ed) The microcomputer in cell and neurobiology research. Elsevier, New York, pp 265–292

    Google Scholar 

  • Levinthal C, Ware R (1972) Three dimensional reconstruction from serial sections. Nature 236:207–210

    Article  Google Scholar 

  • Lillie FR (1908) In: Hamilton HL (1952) Lillie’s Development of the Chick. Holt and Co, New York, p 470

  • Lorensen WE, Cline HE (1987) Marching cubes: a high resolution 3D surface construction algorithm. Comput Graph 21:163–169

    Article  Google Scholar 

  • Majlof L, Forsgren P-O (1993) Confocal microscopy: important consideration for accurate imaging. In: Matsumoto B (eds) Methods in cell biology, vol 38. Cell biological applications of confocal microscopy. Academic, New York

  • Manconi F, Markham R, Cox G, Kable E, Fraser IS (2001) Computer-generated, three-dimensional reconstruction of histological parallel serial sections displaying microvascular and glandular structures in human endometrium. Micron 32:449–453

    Article  PubMed  CAS  Google Scholar 

  • Murphy KM, Zalik SE (1999) Endogenous galectins and effect of galectin hapten inhibitors on the differentiation of the chick mesonephros. Dev Dyn 215:248–263

    Article  PubMed  CAS  Google Scholar 

  • Nelsen, OE (1953) Comparative embryology of the vertebrates. Blakiston Comp, New York, pp 777–782

    Google Scholar 

  • Oldmixon EH, Carlsson K (1993) Methods for large data volumes from confocal scanning laser microscopy of lung. J Microsc 170:221–228

    PubMed  CAS  Google Scholar 

  • Papadimitriou C, Yapijakis C, Davaki P (2004) Use of truncated pyramid representation methodology in three-dimensional reconstruction: an example. J Microsc 214:70–75

    Article  PubMed  MathSciNet  CAS  Google Scholar 

  • Pentecost JO, Icardo J, Thornburg KL (1999) 3D computer modeling of human cardiogenesis. Comput Med Imaging Graph 23:45–49

    Article  PubMed  CAS  Google Scholar 

  • Pentecost JO, Sahn DJ, Thornburg BL, Gharib M, Baptista A, Thornburg KL (2001) Graphical and stereolitographic models of the developing human heart lumen. Comput Med Imaging Graph 25:459–463

    Article  PubMed  CAS  Google Scholar 

  • Scarborough J, Aiton JF, McLachlan JC, Smart SD, Whiten SC (1997) The study of early human embryos using interactive 3-dimensional computer reconstructions. J Anat 191(Pt1):117–122

    Article  PubMed  Google Scholar 

  • Sheppard CJR, Török, P (1997) Effects of specimen refractive index on confocal imaging. J Microsc 185:366–374

    Article  Google Scholar 

  • Shumway W, Adamstone FB (1954) Introduction to vertebrate embryology. Wiley, New York, p 208

    Google Scholar 

  • Čapek M, Krekule I (1999) Alignment of adjacent picture frames captured by a CLSM. IEEE Trans Inform Technol Biomed 3:119–124

    Article  Google Scholar 

  • Vazquez M-D, Bouchet P, Mallet J-L, Foliquet B, Gérard H, LeHeup B (1998) 3D reconstruction of the mouse mesonephros. Anat Histol Embryol 27:283–287

    Article  PubMed  CAS  Google Scholar 

  • Young AA, Legrice IJ, Young MA, Smaill BH (1998) Extended confocal microscopy of myocardial laminae and collagen network. J Microsc 192:139–150

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to Mrs. I. Maršálková and Dr. H. Urbanová for their technical assistance. This study was supported by the Ministry of Education of the Czech Republic, project No. 111100005, by the Grant Agency of the Czech Republic, project No. 304/05/0153, by the Grant Agency of Academy of Sciences of the Czech Republic No. B5039302 and by the Academy of Sciences of the Czech Republic (Grant AVOZ No. 5011922).

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Authors and Affiliations

  1. Institute of Histology and Embryology, 1st Faculty of Medicine, Charles University, Albertov 4, 128 01, Prague 2, Czech Republic

    Marie Jirkovská, Tomáš Kučera & Jaroslav Macášek

  2. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 01, Prague 4, Czech Republic

    Iva Náprstková

  3. Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 01, Prague 4, Czech Republic

    Jiří Janáček, Petr Karen & Lucie Kubínová

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  1. Marie Jirkovská
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Correspondence to Marie Jirkovská.

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Jirkovská, M., Náprstková, I., Janáček, J. et al. Three-dimensional reconstructions from non-deparaffinized tissue sections. Anat Embryol 210, 163–173 (2005). https://doi.org/10.1007/s00429-005-0006-8

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