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Histochemical localization of cholinesterase activity in the dental epithelium of guinea pig teeth

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Abstract

Cholinesterase is known for its remarkable diversity in distribution and function. An association of this enzyme with proliferative and morpho-differentiating tissues has been reported in several species. Here we report on the first evidence of the presence of cholinesterase in the enamel organ of continuously erupting incisors and molars of the guinea pig. Frozen sections of the incisors and molars of the guinea pig were incubated for histochemical demonstration of cholinesterase activity by means of the thiocholine method as described by Karnovsky and Root. The cholinesterase activity was observed in several types of cells of the dental epithelium; cells forming the basal portion of the enamel organ, outer enamel epithelium and maturation stage ameloblasts of both the incisors and molars. In the crown analogue side, the outer enamel epithelial cells gained strong reactions for cholinesterase and maintained the reaction throughout the secretory and maturation stages of amelogenesis. In contrast, cholinesterase reactions were lacking in the inner enamel epithelium, pre-ameloblasts, and secretory ameloblasts. In the early stage of enamel maturation, ameloblasts began to show positive reactions for cholinesterase, which was upregulated in the incisal direction. Although both tooth types showed similar reactive patterns for cholinesterase at the growing ends, maturation ameloblasts depicted a different pattern of staining displaying the reactions only sporadically in molars. These data indicate the role of cholinesterase in the enamel organ in tooth morphogenesis and function of guinea pig teeth.

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Acknowledgements

This study was supported in part by grants-in-aid for scientific research from Japan Society for the Promotion of Science (12557151, 14370577).

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Authors and Affiliations

  1. Department of Basic Sciences, Faculty of Dental Sciences, University of Peradeniya, Sri Lanka

    C. K. Jayawardena

  2. Biostructural Science, Division of Biomatrix, Department of Hard Tissue Engineering, Graduate School of Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113-8549, Tokyo, Japan

    C. K. Jayawardena & Y. Takano

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  1. C. K. Jayawardena
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  2. Y. Takano
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Correspondence to Y. Takano.

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Jayawardena, C.K., Takano, Y. Histochemical localization of cholinesterase activity in the dental epithelium of guinea pig teeth. Anat Embryol 208, 281–287 (2004). https://doi.org/10.1007/s00429-004-0397-y

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  • DOI: https://doi.org/10.1007/s00429-004-0397-y

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