Skip to main content
SpringerLink
Log in

Palatal shelf movement during palatogenesis: a fate map of the fetal mouse palate cultured in vitro

  • Original Article
  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Abstract

Day-13 fetal mouse palates (plug day=day 0) were labeled with carbon particles at various sites of palatal shelves and cultivated in a chemically defined medium for up to 48 h. During the culture period, the bilateral palatal shelves came in contact and fused with each other, which simulated in vivo palatogenesis. The carbon study revealed that at the midpalatal region, the medial edge of the palatal shelf elevated to the horizontal plane, elongated toward the midline, and made contact with the medial edge of the opposing shelf. On the other hand, near the anterior and posterior ends of the shelf, some new tissue was formed at the medial edge of the shelf by remodeling and this newly formed tissue took part in palatal fusion. The results of the present study indicate that during mouse palatogenesis, the anterior and posterior regions of the palatal shelf behave differently from the midpalatal region. It seems that in the fetal mouse palate, the midpalate closes mainly by means of rotation and medial elongation of the shelf, whereas the anterior and posterior parts of the palate close mainly by tissue remodeling of the medial edge and partly by medial elongation of the shelf.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–f
Fig. 2a–f
Fig. 3

Similar content being viewed by others

References

  • Amprino R (1976) Aspects of limb morphogenesis in the chicken. In: DeHaan RL, Ursprung H (eds) Organogenesis. Holt, Rinehart and Winston, New York, pp 255–281

  • Amprino R, Ambrosi G (1973) Experimental analysis of the chick embryo limb bud growth. Arch Biol Brux 84:35–86

    Google Scholar 

  • Bowen J, Hincliffe JR, Horder TJ, Reeve AMF (1989) The fate map of the chick forelimb-bud and its bearing on hypothesized developmental control mechanisms. Anat Embryol 179:269–283

    CAS  PubMed  Google Scholar 

  • Brinkley L, Basehoar G, Avery JK (1974) Effects of craniofacial structures on in vitro palatal shelf elevation. J Dent Res 53:63

    Google Scholar 

  • Brinkley LL, Bookstein FL (1986) Cell distribution during mouse secondary palate closure. II. Mesenchymal cells. J Embryol Exp Morph 96:111–130

    CAS  PubMed  Google Scholar 

  • Brinkley LL, Morris-Wiman J (1987) Computer-assisted analysis of hyaluronate distribution during morphogenesis of the mouse secondary palate. Development 100:629–636

    CAS  PubMed  Google Scholar 

  • Carette MJ, Ferguson MW (1992) The fate of medial edge epithelial cells during palatal fusion in vitro: an analysis by DiI labelling and confocal microscopy. Development 114:379–388

    CAS  PubMed  Google Scholar 

  • Coleman RD (1965) Development of the rat palate. Anat Rec 151:107–118

    CAS  PubMed  Google Scholar 

  • Cottrill CP (1983) Fate maps and the chick limb bud: a new method. J Anat 136:617–618

    Google Scholar 

  • Ferguson MWJ (1988) Palate development. Development 103 (Suppl):41–60

    PubMed  Google Scholar 

  • Garton HJ, Schoenwolf GC (1996) Improving the efficacy of fluorescent labeling for histological tracking of cells in early mammalian and avian embryos. Anat Rec 244:112–117

    Article  CAS  PubMed  Google Scholar 

  • Greene RM, Kochhar DM (1973) Spatial relations in the oral cavity of cortisone-treated mouse fetuses during the time of secondary palate closure. Teratology 8:153–162

    CAS  PubMed  Google Scholar 

  • Greene RM, Pratt RM (1976) Developmental aspects of secondary palate formation. J Embryol Exp Morph 36:225–245

    CAS  PubMed  Google Scholar 

  • Griffith CM, Hay ED (1992) Epithelial-mesenchymal transformation during palatal fusion: carboxyfluorescein traces cells at light and electron microscopic levels. Development 116:1087–1099

    CAS  PubMed  Google Scholar 

  • Grove RI, Pratt RM (1984) Influence of epidermal growth factor and cyclic AMP on growth and differentiation of palatal epithelial cells in culture. Dev Biol 106:427–437

    CAS  PubMed  Google Scholar 

  • Hassell JR (1975) The development of rat palatal shelves in vitro. An ultrastructural analysis of the inhibition of epithelial cell death and palate fusion by the epidermal growth factor. Dev Biol 45:90–102

    CAS  PubMed  Google Scholar 

  • Hudson CD, Shapiro BL (1973) An autoradiographic study of deoxyribonucleic acid synthesis in embryonic rat palatal shelf epithelium with reference to the concept of programmed cell death. Arch Oral Biol 18:77–84

    CAS  PubMed  Google Scholar 

  • Knudsen TB, Bullett RF, Zimmerman EF (1985) Histochemical localization of glycosaminoglycans during morphogenesis of the secondary palate in mice. Anat Embryol 173:137–142

    CAS  PubMed  Google Scholar 

  • Kosazuma T, Kawauchi S, Chou M-J, Shiota K (1994) Susceptibility of day-12.5 and day-13.5 fetal mouse palates cultured in vitro to 5-fluorouracil and hydroxyurea. Cong Anom 34:183–191

    Google Scholar 

  • Larsson KS (1961) Studies on the closure of the secondary palate. III. Autoradiographic and histochemical studies in the normal mouse embryo. Acta Morphol Neerlando-Scand 4:349–367

    Google Scholar 

  • Larsson KS (1962) Studies on the closure of the secondary palate. IV. Autoradiographic and histochemical studies of mouse embryos from cortisone-treated mothers. Acta Morphol Neerlando-Scand 4:369–386

    Google Scholar 

  • Larsson KS, Bostrom H, Carlson S (1959) Studies on the closure of the secondary palate. I. Autoradiographic study in the normal mouse embryo. Exp Cell Res 16:379–383

    CAS  PubMed  Google Scholar 

  • Lipton BH, Jacobson AG (1974) Analysis of normal somite development. Dev Biol 38:73–90

    CAS  PubMed  Google Scholar 

  • Martinez-Alvarez C, Tudela C, Perez-Miguelsanz J, O’Kane S, Puerta J, Ferguson MW (2000) Medial edge epithelial cell fate during palatal fusion. Dev Biol 220:343–357

    Article  CAS  PubMed  Google Scholar 

  • Mino Y, Mizusawa H, Shiota K (1994) Effects of anticonvulsant drugs on fetal mouse palates cultured in vitro. Reprod Toxicol 8:225–230

    Article  CAS  PubMed  Google Scholar 

  • Pratt RM (1987) Role of epidermal growth factor in embryonic development. In: Sawyer R (ed) Current topics in developmental biology. Academic Press, New York, vol 22, pp 175–193

  • Pratt RM, Martin GR (1975) Epithelial cell death and cyclic AMP increase during palatal development. Proc Natl Acad Sci USA 72:874–877

    CAS  PubMed  Google Scholar 

  • Ruiz I, Altaba A, Warga RM, Stern C (1993) Fate maps and cell lineage analysis. In: Stern C, Holland P (eds) Essential developmental biology: a practical approach, Oxford University Press, New York, pp 81–95

  • Saunders JW (1948) The proximo-distal sequence of origin of the parts of the chick wing and the role of the ectoderm. J Exp Zool 108:363–402

    Google Scholar 

  • Seichert V (1966) Differential growth pattern in the chick embryo wing-bud between the 3.5th and 7th day of incubation. Folia Morph (Praha) 14:325–333

    Google Scholar 

  • Shiota K, Fujita S, Akiyama T, Mori C (1990a) Expression of the epidermal growth factor receptor in developing fetal mouse palates: an immunohistochemical study. Am J Anat 188:401–408

    CAS  PubMed  Google Scholar 

  • Shiota K, Kosazuma T, Klug S, Neubert D (1990b) Development of the fetal mouse palate in suspension organ culture. Acta Anat 137:59–64

    CAS  Google Scholar 

  • Shuler CF, Guo Y, Majumder A, Luo R (1991) Molecular and morphologic changes during the epithelial-mesenchymal transformation of palatal shelf medial edge epithelium in vitro. Int J Dev Biol 35:463–472

    CAS  PubMed  Google Scholar 

  • Shuler CF, Halpern DE, Guo Y, Sank C (1992) Medial edge epithelium fate by cell lineage analysis during epithelial-mesenchymal transformation in vivo. Dev Biol 154:318–330

    CAS  PubMed  Google Scholar 

  • Spratt NT (1955) Analysis of the organizer center in the early chick embryo. 1. Localization of prospective notochord and somite cells. J Exp Zool 128:121–163

    Google Scholar 

  • Tamarin A (1982) The formation of the primitive choanae and the junction of the primary and secondary palates in the mouse. Am J Anat 165:319–337

    CAS  PubMed  Google Scholar 

  • Tschumi PA (1957) The growth of the hindlimb bud of Xenopus laevis and its dependence upon the epidermis. J Anat 91:149–173

    PubMed  Google Scholar 

  • Walker BE (1961) The association of mucopolysaccharides with morphogenesis of the palate and other structures in mouse embryos. J Embryol Exp Morph 9:22–31

    CAS  PubMed  Google Scholar 

  • Walker BE, Fraser FC (1956) Closure of the secondary palate in three strains of mice. J Embryol Exp Morph 4:176–189

    Google Scholar 

  • Walker BE, Patterson A (1974) The mechanism of cortisone-induced cleft palate. J Dent Res 55:63

    Google Scholar 

  • Wee EL, Wolfson LG, Zimmerman EF (1976) Palate shelf movement in mouse embryo culture: evidence for skeletal and smooth muscle contractility. Dev Biol 48:91–103

    Google Scholar 

  • Yasuda Y, Fujimoto T (1986) The anterior half of mouse palatal shelf is elevated by a remodeling movement. Acta Anat 125:37–41

    CAS  Google Scholar 

  • Zhang Z, Song Y, Zhao X, Zhang X, Fermin C, Chen Y (2002) Rescue of cleft palate in Msx1-deficient mice by transgenic Bmp4 reveals a network of BMP and Shh signaling in the regulation of mammalian palatogenesis. Development 129:4135–4146

    CAS  PubMed  Google Scholar 

  • Zimmerman EF, Clark RL, Ganguli S, Venkatasubramanian K (1983) Serotonin regulation of palatal cell motility and metabolism. J Craniofac Genet Dev Biol 3:371–385

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported in part by grants-in-aid from the Japanese Ministry of Education, Culture, Sports, Science and Technology.

Author information

Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, 606-8501, Kyoto, Japan

    Ming-Jen Chou, Tsuneo Kosazuma, Toshiya Takigawa & Kohei Shiota

  2. Congenital Anomaly Research Center, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Shigehito Yamada, Sachiko Takahara & Kohei Shiota

  3. Department of Anatomy, Chung Shan Medical University, 402, Taichung, Taiwan

    Ming-Jen Chou

Authors
  1. Ming-Jen Chou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tsuneo Kosazuma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshiya Takigawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shigehito Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sachiko Takahara
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kohei Shiota
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kohei Shiota.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chou, MJ., Kosazuma, T., Takigawa, T. et al. Palatal shelf movement during palatogenesis: a fate map of the fetal mouse palate cultured in vitro. Anat Embryol 208, 19–25 (2004). https://doi.org/10.1007/s00429-004-0379-0

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00429-004-0379-0

Keywords

Search

Navigation