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Comparison of histological and molecular features of pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma

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Abstract

Pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma are pediatric B cell lymphomas with similar clinical characteristics but distinct histological features. We investigated the differences between pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma by comparing their histological and molecular characteristics. A total of 5 pediatric-type follicular lymphoma and 11 pediatric nodal marginal zone lymphoma patients were included in the study. In the histological review, 5 of the 16 cases showed overlapping morphological features of pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma; hence, they were reclassified as “mixed type.” In molecular analysis, using panel-based massively parallel sequencing, MAP2K1, TNFRSF14, and IRF8 mutations were found in 6, 3, and 2 of the 11 pediatric nodal marginal zone lymphoma patients, respectively, and IRF8 mutation was found in one of the five pediatric-type follicular lymphoma patients. There were no significant differences in genetic alterations established from the histologically reclassified diagnosis as well as the initial diagnosis. Pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma showed morphological overlap in some cases, and no difference between the two was found upon molecular analysis. These findings suggest the possibility that pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma are single entity pediatric B-cell lymphoma with broad morphological spectrum.

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Authors and Affiliations

  1. Department of Pathology, Korea University Guro Hospital, Seoul, Korea

    Jiyeon Lee

  2. Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, #81, Irwon-ro, Gangnam-Gu, Seoul, 06351, Korea

    Jiyeon Lee & Junhun Cho

  3. Department of Pathology, Ajou University School of Medicine, Suwon, Korea

    Jae-Ho Han

  4. Department of Pathology, Pusan National University Hospital, Pusan National University School of Medicine, Busan, Korea

    Chang Hun Lee

  5. Department of Pathology, Jeonbuk National University Hospital, Jeonbuk National University Medical School, Jeonju, Korea

    Ho-Sung Park

  6. Department of Pathology, Hallym University Sacred Heart Hospital, Anyang, Korea

    Soo Kee Min

  7. Department of Pathology, Nowon Eulji Medical Center, Eulji University, Seoul, Korea

    Hojung Lee

  8. Department of Pathology, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

    Uiju Cho

  9. Division of Hematology and Oncology, Department of Medicine Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

    Sang Eun Yoon, Seok Jin Kim & Won Seog Kim

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Contributions

J. L. contributed to study design, data interpretation, and data analysis; created the figures; and led the write-up; J.-H. H., C. H. L., H.-S. P., S. K. M., H. L., and U. C. contributed to data analysis; S. E. Y., S. J. K, and W. S. K. contributed to data interpretation and data analysis; J. C. oversaw all aspects of study design, data interpretation, and the write-up.

Corresponding author

Correspondence to Junhun Cho.

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Ethics approval and consent to participate

All methods were carried out in accordance with Helsinki declaration, and all protocols of this study were approved by the Institutional Review Board of Samsung Medical Center (IRB file number: SMC 2021-02-066-003).

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The authors declare no competing interests.

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The original online version of this article was revised due to updated affiliation name

Supplementary Information

Supplementary file 1

Supplementary Table 1. The list of antibodies used for immunohistochemistry in this stud, Supplementary Table 2. Gene list included in HemaSCAN, Supplementary Table 3. The ratio of FoxP1-positive cells in the intrafollicular and extrafollicular areas of each case measured through digital image analysis (QuPath), Supplementary Table 4. FoxP1 positive rate according to reclassified diagnosis, Supplementary Table 5. Detailed information on genetic mutations of all 16 patients.

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Lee, J., Han, JH., Lee, C.H. et al. Comparison of histological and molecular features of pediatric-type follicular lymphoma and pediatric nodal marginal zone lymphoma. Virchows Arch 482, 849–858 (2023). https://doi.org/10.1007/s00428-023-03493-x

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