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Virchows Archiv

, Volume 472, Issue 3, pp 383–393 | Cite as

Distinct histopathological characteristics in colorectal submucosal invasive carcinoma arising in sessile serrated adenoma/polyp and conventional tubular adenoma

  • Takashi Murakami
  • Hiroyuki Mitomi
  • Takashi Yao
  • Tsuyoshi Saito
  • Tomoyoshi Shibuya
  • Naoto Sakamoto
  • Taro Osada
  • Sumio Watanabe
Original Article

Abstract

The histopathological characteristics of colorectal submucosal invasive carcinoma arising in sessile serrated adenoma/polyp (SSA/P), a rare malignant tumour, have not yet been fully elucidated. To investigate the features of such, we retrospectively analysed 40 submucosal invasive carcinomas with SSA/P (CA-SSA/P) and compared them to 129 cases of submucosal invasive carcinoma with conventional tubular adenoma (CA-AD). We additionally performed hMLH1 immunostaining. CA-SSA/Ps were significantly smaller than CA-ADs (P < 0.001). Histologically, well to moderately differentiated adenocarcinoma was predominant in both CA-SSA/Ps and CA-ADs. No significant differences in depth of invasion were found between the two groups. However, lymphatic invasion was more often found in CA-SSA/Ps (30%) than in CA-ADs (13%; P = 0.028), as was lymph node metastasis (CA-SSA/Ps, 28%; CA-ADs, 7%; P = 0.011). Furthermore, mucinous component and serrated architecture were significantly more frequent in CA-SSA/Ps (30 and 63%) than in CA-ADs (5 and 18%; P < 0.001, respectively). Tumour-infiltrating lymphocytes and Crohn-like inflammatory reaction were also more frequently found in CA-SSA/Ps (70 and 30%) than in CA-ADs (31 and 9%; P ≤ 0.001, respectively), whereas the opposite was true of desmoplastic reaction (CA-SSA/Ps, 35%; CA-ADs, 67%; P < 0.001). Loss of hMLH1 expression was more frequent in CA-SSA/P cases (93%) than in CA-AD cases (5%; P < 0.001). In conclusion, CA-SSA/P lesions exhibit a higher potential for lymphatic invasion and lymph node metastasis and have distinct histopathological features, including mucinous component, serrated architecture, tumour-infiltrating lymphocytes, Crohn-like inflammatory reaction, and absence of desmoplastic reaction, compared to their conventional counterparts.

Keywords

Sessile serrated adenoma/polyp Submucosal invasive carcinoma Serrated neoplasia pathway Conventional adenoma-carcinoma sequence Histopathology 

Notes

Acknowledgments

The authors thank Mrs. Keiko Mitani for her assistance with histology. The work was supported in part by a Grant-in-Aid from the Japan Society for the Promotion of Science (#24590429 to H. Mitomi and #15K19059 to T. Murakami).

Funding

This work was supported in part by a Grant-in-Aid from the Japan Society for the Promotion of Science (#24590429 to H. Mitomi and #15K19059 to T. Murakami).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

This study was approved by the Institutional Review Board and the ethical committee of our hospital (registration #2012015). For this retrospective study, no informed consent was required.

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Copyright information

© Springer-Verlag GmbH Deutschland 2017

Authors and Affiliations

  1. 1.Department of GastroenterologyJuntendo University School of MedicineTokyoJapan
  2. 2.Department of Human PathologyJuntendo University School of MedicineTokyoJapan
  3. 3.Department of Diagnostic Pathology and Laboratory MedicineOdawara Municipal HospitalOdawaraJapan

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