Virchows Archiv

, Volume 469, Issue 1, pp 81–91 | Cite as

Unclassified renal cell carcinoma with tubulopapillary architecture, clear cell phenotype, and chromosome 8 monosomy: a new kid on the block

  • Thanh T. H. Lan
  • Jennifer Keller-Ramey
  • Carrie Fitzpatrick
  • Sabah Kadri
  • Jerome B. Taxy
  • Jeremy P. Segal
  • Larissa V. Furtado
  • Tatjana Antic
Original Article


Accurate subtyping of renal cell carcinomas (RCCs) has become clinically important for therapy and prognostication. RCC subtypes are defined by distinct morphologic and immunohistochemical profiles, and in some instances recurrent cytogenetic and molecular properties. However, some tumors exhibit overlapping morphologic and immunophenotypic features, frequent enough to pose diagnostic dilemmas. This report concerns six histologically unusual RCCs that showed tubulopapillary architecture, clear cell phenotype, and non-diagnostic immunohistochemical profiles. Further investigation of these tumors utilized a single nucleotide polymorphism (SNP) microarray platform (OncoScan®, Affymetrix) that employed molecular inversion probe (MIP) technology to investigate genome-wide chromosomal copy number changes and loss of heterozygosity in formalin-fixed paraffin-embedded sections. The six tumors were assayed in parallel with and in comparison to RCC with typical morphologic or immunohistochemical features for a specific subtype (clear cell, clear cell papillary, and microphthalmia transcription factor (MiT) family translocation RCC). Three of the unusual RCCs showed a molecular signature of clear cell RCC and one of papillary RCC. The remaining two showed monosomy of chromosome 8. Those two cases were tested via next-generation sequencing, and no pathogenic variants were detected, including those in the genes VHL, PBRM1, SETD2, KDM5C, or BAP1. The addition of molecular investigations such as reported here as applied to histologically and immunohistochemically unusual RCC may help to define additional subtypes and contribute to the development of targeted therapy for renal cancer.


Renal cell carcinoma The OncoScan® FFPE assay Next-generation sequencing Chromosome 8 monosomy 



The authors would like to thank Bradley C. Long for the help with this project.

Compliance with ethical standards

This study was approved by the Institutional Review Board at the University of Chicago and was carried out in accordance with the guidelines for archived specimens and patient chart review. The authors do not have any conflict of interest or disclosures and are in compliance with ethical standards. No funding was used in this study.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Thanh T. H. Lan
    • 1
  • Jennifer Keller-Ramey
    • 1
  • Carrie Fitzpatrick
    • 1
  • Sabah Kadri
    • 1
    • 2
  • Jerome B. Taxy
    • 3
  • Jeremy P. Segal
    • 1
  • Larissa V. Furtado
    • 1
  • Tatjana Antic
    • 1
  1. 1.Department of PathologyThe University of ChicagoChicagoUSA
  2. 2.Center for Research InformaticsThe University of ChicagoChicagoUSA
  3. 3.Department of Pathology and Laboratory MedicineNorthShore University HealthSystemEvanstonUSA

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