Virchows Archiv

, Volume 467, Issue 2, pp 185–191 | Cite as

Genetic testing of leiomyoma tissue in women younger than 30 years old might provide an effective screening approach for the hereditary leiomyomatosis and renal cell cancer syndrome (HLRCC)

  • Petr Martínek
  • Petr Grossmann
  • Ondřej Hes
  • Jiří Bouda
  • Viktor Eret
  • Norma Frizzell
  • Anthony J Gill
  • Ondrej Ondič
Original Article


We have studied the viability of targeted molecular screening for the identification of female patients with hereditary leiomyomatosis and renal cell cancer (HLRCC) syndrome. Affected patients harbor a germ-line heterozygous mutation of the fumarate hydratase (FH) gene. Clinically, some patients present with aggressive renal cell carcinoma. Concerning women, in almost all cases, this is preceded by symptomatic uterine leiomyoma. We aimed to identify women operated on for symptomatic leiomyoma by the age of 30. Archived paraffin-embedded leiomyoma tissue was tested for the FH gene mutation in 14 cases. Two patients with multiple leiomyomas and with the confirmed germ-line mutations c.1433_1434dupAAA, p.(Lys477dup) and c.953A>T, p.(His318Leu) were identified and enrolled in a surveillance program. Statistically significant correlation between the presence of multiple uterine leiomyomas (more than seven in our experience) and the FH gene mutation was found. The immunohistochemical expression pattern, of simultaneous FH absence and S-(2-succino)cysteine (2SC) positivity, correlated with the results of the molecular genetic study in only one case. The histomorphologically simultaneous detection of enlarged nucleoli with a clear halo of leiomyocyte nuclei, their fibrillary cytoplasm, the presence of eosinophilic globules, and staghorn vessels proved to be only a partially sensitive indicator of HLRCC-associated leiomyoma and fully correlated with immunohistochemistry and molecular genetic study only in one case. Molecular genetic testing is presently the only reliable diagnostic tool able to identify HLRCC patients. The sensitivity and specificity of the presence of multiple leiomyomas in women with the FH gene mutation who are younger than 30 years old should be confirmed in larger scale studies. The applied targeted molecular screening protocol proved to be effective, resulting in identification of two positive patients out of fourteen tested individuals.


HLRCC Fumarate hydratase FH Leiomyoma Renal cell carcinoma 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Petr Martínek
    • 1
    • 2
  • Petr Grossmann
    • 2
  • Ondřej Hes
    • 1
  • Jiří Bouda
    • 3
  • Viktor Eret
    • 4
  • Norma Frizzell
    • 5
  • Anthony J Gill
    • 6
    • 7
  • Ondrej Ondič
    • 1
    • 2
  1. 1.Šikl’s Department of Pathology, University Hospital, Faculty of Medicine in PilsenCharles University in PraguePilsenCzech Republic
  2. 2.Department of GeneticsBioptická laboratoř s.r.oPilsenCzech Republic
  3. 3.Department of Gynecology and Obstetrics, University Hospital, Faculty of Medicine in PilsenCharles University in PraguePilsenCzech Republic
  4. 4.Department of Urology, University Hospital, Faculty of Medicine in PilsenCharles University in PraguePilsenCzech Republic
  5. 5.Department of Pharmacology, Physiology and Neuroscience, School of MedicineUniversity of South CarolinaColumbiaUSA
  6. 6.Department of Anatomical PathologyRoyal North Shore HospitalSt LeonardsAustralia
  7. 7.University of SydneySydneyAustralia

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