Abstract
The biological behavior of gastrointestinal stromal tumors (GISTs) ranges from benign to malignant, and the risk of an adverse outcome is correlated with the location of the primary tumor, tumor size, and mitotic counts. Cell cycle regulators are potentially associated with the tumorigenesis and progression of GISTs. Checkpoint with forkhead and ring finger (CHFR) functions as an important checkpoint protein in the early to mid-prophase to regulate mitosis. In this study, we evaluated the expression of CHFR and several cell cycle regulators, including cyclin A, cyclin B1, cdc2, and cdk2, by immunohistochemical staining in 53 cases of primary gastric GISTs, and compared the immunohistochemical results with the clinicopathological factors or the GIST risk grades as modified by Miettinen et al. Of the 53 cases, 18 (34%) showed decreased nuclear CHFR expression. Decreased CHFR expression was correlated with higher mitotic counts [>5/50 high-power fields (HPFs)] (p = 0.039) and a high-risk grade (p = 0.0475), but not with expression of other cell cycle regulators. Higher cyclin A labeling index (LI, >1.5%), cyclin B1 LI (>0.25%), cdc2 LI (>1.16%), Ki-67 LI (>4.9%), mitotic counts (>5/50 HPF) and high-risk grade were each associated with shorter disease-free survival (p = 0.0017, p = 0.003, p = 0.0471, p = 0.002, p < 0.001, and p = 0.0017, respectively). Our results suggest that modified risk grade and increased expression of G2–M regulators such as cyclin A, cyclin B1, and cdc2 are useful for predicting the biological behavior of gastric GISTs. In addition, decreased CHFR expression may play a role in increased proliferative activity of higher grade GISTs.
Similar content being viewed by others
References
Fletcher CD, Bermann JJ, Corless C et al (2002) Diagnosis of gastrointestinal stromal tumors: a consensus approach. Hum Pathol 33:459–465
Miettinen M, Lasota J (2006) Gastrointestinal stromal tumors: pathology and prognosis at different sites. Semin Diagn Pathol 23:70–83
Lasota J, Miettinen M (2006) KIT and PDGFRA mutations in gastrointestinal stromal tumors (GISTs). Semin Diagn Pathol 23:91–102
Hirota S, Isozaki K, Moriyama Y et al (1998) Gain-of function mutations of c-kit in human gastrointestinal stromal tumors. Science 279:577–580
Heinrich MC, Corless CL, Duensing A et al (2003) PDGFRA activating mutations in gastrointestinal stromal tumors. Science 299:708–710
Hirota S, Ohashi A, Nishida T et al (2003) Gain-of function mutations of platelet-derived growth factor receptor alpha gene in gastrointestinal stromal tumors. Gastroenterology 125:660–667
Yamamoto H, Oda Y, Kawaguchi K et al (2004) c-kit and PDGFRA mutations in extragastrointestinal stromal tumor (gastrointestinal stromal tumor of the soft tissue). Am J Surg Pathol 28:479–488
Miettinen M, Sobin LH, Lasota J (2005) Gastrointestinal stromal tumors of the stomach. A clinicopathologic, immunohistochemical, and molecular genetic study of 1765 cases with long-term follow up. Am J Surg Pathol 29:52–68
Schimieder M, Wolf S, Danner B et al (2008) p16 expression differentiates high-risk gastrointestinal stromal tumor and predicts poor outcome. Neoplasia 10(10):1154–1625
Schneider-Stock R, Boltze C, Lasota J et al (2003) High prognostic value of p16INK4 alterations in gastrointestinal stromal tumors. J Clin Oncol 21(9):1688–1697
Haller F, Lobke C, Ruschhaoupt M et al (2008) Loss of 9p leads to p16INK4A down-regulation and enables RB/E2F1-dependent cell cycle promotion in gastrointestinal stromal tumors (GISTs). J Pathol 215:253–262
Scolnick DM, Halazonetis TD (2000) Chfr defines a mitotic stress check-point that delays entry into metaphase. Nature 406:430–435
Summes MK, Bothos J, Halazonetis TD (2005) The CHFR mitotic checkpoint protein delays cell cycle progression by excluding cyclin B1 from the nucleus. Oncogene 24:2589–2598
Kang D, Chen J, Wong J et al (2002) The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition. J Cell Biol 56:249–259
Ogi K, Toyota M, Mita H et al (2005) Small interfering RNA-induced CHFR silencing sensitizes oral squamous cell cancer cells to microtubule inhibitors. Cancer Biol Ther 4:773–780
Satoh A, Toyota M, Itoh F et al (2003) Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer. Cancer Res 63:8606–8613
Mizuno K, Osada H, Konishi H et al (2002) Aberrant hypermethylation of the CHFR prophase checkpoint gene in human lung cancers. Oncogene 21:2328–2333
Erson AE, Petty EM (2004) CHFR-associated early G2/M checkpoint defects in breast cancer cells. Mol Carcinog 39:26–33
Nakamura N, Yamamoto H, Yao T et al (2005) Prognostic significance of abnormalities of cell-cycle regulatory proteins in gastrointestinal stromal tumor and relevance of the risk-grading system. Hum Pathol 36:828–837
Kobayashi C, Oda Y, Takahira T et al (2006) Aberrant expression of CHFR in malignant peripheral nerve sheath tumors. Mod Pathol 19:524–532
Daniels MJ, Marson A, Venkitaraman AR (2004) PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein. Nat Struct Mol Biol 11:1114–1121
Toyota M, Sasaki Y, Satoh A et al (2003) Epigenetic inactivation of CHFR in human tumors. Proc Nati Acad Sci U S A 100:7818–7823
Igarashi S, Suzuki H, Niinuma T et al (2010) A novel correlation between LINE-1 hypomethylation and the malignancy of gastrointesinal stromal tumors. Clin Cancer Res 16:5114–4123
Soutto M, Peng D, Razvi M et al (2010) Epigenetic and genetic silencing of CHFR in esophageal adenocarcinomas. Cancer 116:4033–4042
Marumoto T, Hirota T, Morisaki T et al (2002) Roles of aurora-A kinase in mitotic entry and G2 checkpoint in mammalian cells. Genes Cells 7:1173–1182
Hirota T, Kunitoku N, Sasayama T et al (2003) Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells. Cell 114:585–598
Acknowledgment
This study was supported in part by the Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, Tokyo, Japan (Grant Number 22790346; H. Yamamoto).
Conflict of interest statement
We declare that we have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fujita, A., Yamamoto, H., Imamura, M. et al. Expression level of the mitotic checkpoint protein and G2–M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach. Virchows Arch 460, 163–169 (2012). https://doi.org/10.1007/s00428-011-1181-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00428-011-1181-z