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Tyrosine phosphatase SHP-1 is expressed higher in multisystem than in single-system Langerhans cell histiocytosis by immunohistochemistry

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Abstract

Langerhans cell histiocytosis (LCH) is a proliferative disorder of Langerhans cell (LC)-like CD1a-positive cell (LCH cell) with unknown causes. LCH consists of two subtypes: single-system LCH (LCH-SS) with favorable prognosis and multisystem LCH (LCH-MS) with poor prognosis. LCH has been indicated as a neoplastic disorder from monoclonal characteristics of LCH cells. This study aimed to investigate an expression of tyrosine phosphatase SHP-1 in LCH, since its expression levels were variously reported in many tumors, overexpression in ovarian cancers (a candidate oncoprotein), and downregulation by methylation in gastric cancers, prostate cancers, malignant lymphomas, and leukemias (a putative tumor suppressor). By immunohistochemistry (IHC), the SHP-1 expression in LCs and LCH cells was compared in LCH (two subtypes: LCH-SS = 21, LCH-MS = 12), dermatopathic lymphadenopathy (DLA) (n = 9) and normal epidermal LCs (n = 3) near LCH lesion. IHC results were analyzed semiquantitatively using a Photoshop software. The mean intensity score (IS) of DLA, LCH-SS, LCH-MS, and LCs were 47, 100, 139, and 167 (in arbitrary unit), respectively. The IS had significant differences among LCH-SS, LCH-MS, and DLA (p < 0.01). SHP-1 is expressed significantly higher in LCH-MS than in LCH-SS. SHP-1 can be a progression marker of LCH. SHP-1 is also useful for differential diagnosis between LCH in lymph nodes and DLA.

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Abbreviations

B-ALL:

B lymphoblastic leukemia

CMYK:

Cyan/magenta/yellow/key

IF:

Immunofluorescence

IHC:

Immunohistochemistry

IS:

Intensity score

ISs:

Intensity scores

LC:

Langerhans cell

LCH:

Langerhans cell histiocytosis

LCH cell:

Langerhans cell-like CD1a-positive cell

LCH-MS:

Multisystem LCH

LCH-SS:

Single-system LCH

LCS:

LC sarcoma

meV:

Viable motheaten mice

MHC:

Major histocompatibility complex

PTPN6:

Tyrosine–protein phosphatase nonreceptor type 6

SHP-1:

Src homology region 2 domain-containing phosphatase 1

SHPS-1:

Substrate of SHP-1

T-ALL:

T lymphoblastic leukemia

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Acknowledgments

This work was partly supported by a grant from Hamazaki Cancer Fund. We thank Dr. Daiichiro Hasegawa (Hyogo Children’s Hospital), Dr. Chihiro Shimazaki (Kyoto Prefectural University of Medicine), Dr. Nobuyo Yoshida (Kagoshima University Graduate School of Medicine and Dental Sciences), Dr. Teruhisa Koyama (NHO Okayama Medical Center), Dr. Sanae Kawakami (Ehime University School of Medicine), Dr. Takashi Kanazawa (Gunma University School of Medicine), Dr. Toshinori Hori (Aichi Medical University), Dr. Takashi Sato (Hiroshima University), Dr. Yoshiyuki Kosaka (Hyogo Children’s Hospital), Dr. Chihaya Imai (Niigata University), Dr. Banryoku Higuchi (Nara Medical University), Dr. Keigo Hamahata (Japanese Wakayama Red Cross Medical Center), Dr. Yutaka Kobayshi (Kyoto Second Red Cross Hospital), Dr. Ritsuo Nishiuchi (Kochi Medical Center), Dr. Yasuo Horikoshi (Shizuoka Prefectural Children’s Hospital), Dr. Yoshihiko Hoshida (Sumitomo Hospital), Dr. Kunimitsu Kawahara (Osaka Prefectural Medical Center for Respiratory and Allergic Diseases), Dr. Ichiro Yamadori (NHO Okayama Medical Center), Dr. Nobuya Ohara (Okayama Red Cross Hospital), Dr. Yasushi Horie (Tottori University Hospital), Dr. Francis Jaubert (Hôpital Necker-Enfants-Malades), Dr. Takehiro Tanaka, Dr. Koichi Ichimura, and Dr. Hiroyuki Yanai (Okayama University Hospital) for providing clinical information and specimens and Dr. Kenji Yokota (Okayama University Graduate School of Health Sciences) and the Central Research Laboratory, Okayama University Medical School for the help with the microscopes and software.

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Faculty of Medicine, Division of Molecular Pathology, Tottori University, 86 Nishi-cho Yonago-shi, Tottori, 683–0834, Japan

    Ichiro Murakami, Satoshi Kuwamoto, Masako Kato & Kazuhiko Hayashi

  2. Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Takashi Oka & Tadashi Yoshino

  3. Inserm U90 and U507, Hôpital Necker-Enfants-Malades, Paris, France

    Jean Gogusev

  4. Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Toshihiko Imamura

  5. Department of Pediatrics, Jichi Medical University School of Medicine, Shimotsuke, Japan

    Akira Morimoto

  6. Division of Pediatrics and Hematology, Takasago-seibu Hospital, Takasago, Japan

    Shinsaku Imashuku

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  1. Ichiro Murakami
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  2. Takashi Oka
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  3. Satoshi Kuwamoto
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  9. Shinsaku Imashuku
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  10. Tadashi Yoshino
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Correspondence to Ichiro Murakami.

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Murakami, I., Oka, T., Kuwamoto, S. et al. Tyrosine phosphatase SHP-1 is expressed higher in multisystem than in single-system Langerhans cell histiocytosis by immunohistochemistry. Virchows Arch 459, 227–234 (2011). https://doi.org/10.1007/s00428-011-1090-1

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  • DOI: https://doi.org/10.1007/s00428-011-1090-1

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