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Murine immune response induced by Leishmania major during the implantation of paraffin tablets

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Abstract

We carried out a model of chronic inflammation using a subcutaneous paraffin tablet in mice experimentally infected with Leishmania major. It was previously reported that the parasite load following paraffin implantation occurred at a peak of 21 days in both BALB/c and C57BL/6 mice. At the present study, we have investigated what cytokines and chemokines are directly related to the parasite load in C57BL/6 mice. All mice were divided in four groups: mice implanted with paraffin tablets; mice experimentally infected with L. major; mice implanted with paraffin tablets and experimentally infected with L. major; and mice submitted only to the surgery were used for the Real-Time Polymerase Chain Reaction (RT-PCR) controls. Fragments of skin tissue and the tissue surrounding the paraffin tablets (inflammatory capsule) were collected for histopathology and RT-PCR studies. By 21 days, a diffuse chronic inflammatory reaction was mainly observed in the deep dermis where macrophages parasitized with Leishmania amastigotes were also found. RT-PCR analysis has shown that BALB/c mice showed strong IL-4 and IL-10 mRNA expression than controls with very little expression of IFN-γ. In contrast, both IFN-γ and IL-10 mRNA was found in higher levels in C57BL/6 animals. Moreover, in C57BL/6 mice the expression of chemokines mRNA of CCL3/MIP-1α was more highly expressed than CCL2/MCP-1. We conclude that the Th1 immune response C57BL/6 did not change to a Th2 response, even though C57BL/6 animals presented higher parasitism than BALB/c mice 21 days after infection and paraffin implantation.

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Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Departamento de Patologia Geral, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Av Antonio Carlos, Campus Pampulha, Belo Horizonte, 6627, MG, Brazil

    Maria Letícia Costa Reis, Vanessa Martins Ferreira & Wagner Luiz Tafuri

  2. Department of Cell Biology and Molecular Genetics, Univeristy of Maryland, Microbiology Bldg, College Park, MD, 20742, USA

    Xia Zhang & David M. Mosser

  3. Instituto de Ciências Exatas e Biológicas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto, Morro do Cruzeiro, Bauxita, Ouro Preto, MG, Brazil

    Ricardo Gonçalves

  4. Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Av Antonio Carlos, Campus Pampulha, Belo Horizonte, 6627, MG, Brazil

    Leda Quércia Vieira

  5. Departamento de Anatomia Patológica e Medicina Legal, Universidade Federal de Minas Gerais, Faculdade de Medicina, Av Alfredo Balena 90, Belo Horizonte, MG, Brazil

    Washington Luiz Tafuri

Authors
  1. Maria Letícia Costa Reis
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  2. Vanessa Martins Ferreira
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  8. Wagner Luiz Tafuri
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Correspondence to Wagner Luiz Tafuri.

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Letícia Costa Reis, M., Martins Ferreira, V., Zhang, X. et al. Murine immune response induced by Leishmania major during the implantation of paraffin tablets. Virchows Arch 457, 609–618 (2010). https://doi.org/10.1007/s00428-010-0974-9

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  • DOI: https://doi.org/10.1007/s00428-010-0974-9

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