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Immunophenotyping and oncogene amplifications in tumors of the papilla of Vater

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Abstract

Carcinomas of the ampulla of Vater are rare and assumed to generally arise from preexisting adenomas (adenoma–carcinoma sequence). Histologically, distinct subtypes can be distinguished that were shown to differ significantly in terms of clinical outcome. Since pathologists usually receive bioptic tissue samples of ampullary tumors obtained during endoscopy, accurate classification of carcinoma subtypes can sometimes be difficult on morphological criteria alone. We therefore performed immunohistochemistry using a panel of established marker proteins (CK7, CK20, p21, p27, ESA, bax, and ephrin-B2) on 175 carcinoma, 111 adenoma, and 152 normal mucosa specimens of the ampulla of Vater and identified distinct immunoprofiles for every carcinoma subtype. Fluorescence in situ hybridization analyses of therapeutic target genes (c-myc, EGFR1, CCND1, HER2) found CCND1 to represent the most frequently amplified gene in our series (7.5%).

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Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Institute of Pathology, University of Basel, Schönbeinstrasse 40, 4003, Basel, Switzerland

    Daniel Baumhoer, Inti Zlobec, Luigi Tornillo & Luigi M. Terracciano

  2. Institute of Pathology, University of Regensburg, Regensburg, Germany

    Wolfgang Dietmaier & Petra Ruemmele

  3. Institute of Pathology, Municipal Hospitals of Nuremberg, Nuremberg, Germany

    Peter H. Wuensch

  4. Institute of Pathology, University of Erlangen, Erlangen, Germany

    Arndt Hartmann

  5. Anatomic Pathology Unit, Department of Human Morphology, University of Insubria, Varese, Italy

    Fausto Sessa

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  1. Daniel Baumhoer
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  2. Inti Zlobec
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  3. Luigi Tornillo
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  4. Wolfgang Dietmaier
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  5. Peter H. Wuensch
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  6. Arndt Hartmann
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  7. Fausto Sessa
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  8. Petra Ruemmele
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  9. Luigi M. Terracciano
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Corresponding author

Correspondence to Daniel Baumhoer.

Additional information

Petra Ruemmele and Luigi M. Terracciano shared senior authorship.

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Baumhoer, D., Zlobec, I., Tornillo, L. et al. Immunophenotyping and oncogene amplifications in tumors of the papilla of Vater. Virchows Arch 453, 579–588 (2008). https://doi.org/10.1007/s00428-008-0669-7

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  • DOI: https://doi.org/10.1007/s00428-008-0669-7

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