Abstract
Three-dimensional (3D) visualisation of microscopic structures provides useful information about their configuration and the spatial (hence functional) relationship between different components in tissues. This paper describes 3D dynamic reconstructions of the pre-lymphatic labyrinth in two cases of “normal” breast core biopsies and one case of pseudoangiomatous stromal hyperplasia. Direct anastomoses between pre-lymphatic channels and true lymphatics of the breast were demonstrated. It is concluded that pre-lymphatics are a way of communication between breast epithelial/stromal structures and the main lymphatic system. The present findings suggest that the existence of pre-lymphatics has to be taken in consideration in the intramammary spread of malignant tumours.
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References
Casley-Smith JR, Florey HW (1961) The structure of normal small lymphatics. Q J Exp Physiol Cogn Med Sci 46:101–106
Casley-Smith JR (1973) The lymphatic system in inflammation. In: Zweifach BW, Grant L, McCluskey RT (eds) The inflammatory process. Academic, New York, pp 161–203
Hartveit F (1990) Attenuated cells in breast stroma: the missing lymphatic system of the breast. Histopathology 16:533–543
Eusebi V, Tavassoli FA (2008) Tumors of the breast. American Registry of Pathology/AFIP, Washington
Ozzello L, Sanpitak P (1970) Epithelial-stromal junction of intraductal carcinoma of the breast. Cancer 26:1186–1198
Vuitch MF, Rosen PP, Erlandson RA (1986) Pseudoangiomatous hyperplasia of the mammary stroma. Hum Pathol 17:185–191
Badve S, Sloane JP (1994) Pseudoangiomatous hyperplasia of male breast. Histopathology 26:463–466
Damiani S, Peterse JL, Eusebi V (2002) Malignant neoplasms infiltrating “pseudoangiomatous” stromal hyperplasia of the breast: an unrecognized pathway of tumor spread. Histopathology 41:208–215
Bussolati G, Marchiò C, Volante M (2005) Tissue array as fiducial markers for section alignment in 3-D reconstruction technology. J Cell Mol Med 9:438–445
Foschini MP, Papotti M, Parmeggiani A et al (2004) Three-dimensional reconstruction of vessel distribution in benign and malignant lesions of thyroid. Virchows Arch 445:189–198
Foschini MP, Righi A, Cucchi MC et al (2006) The impact of large sections and 3D technique on the study of lobular in situ and invasive carcinoma of the breast. Virchows Arch 448:256–261
Foschini MP, Tot T, Eusebi V (2002) Large-section (macrosection) histologic slides. In: Silverstein MJ (ed) Ductal carcinoma in situ of the breast. Lippincott, Philadelphia, pp 249–254
Wellings SR, Jensen HM (1973) On the origin and progression of ductal carcinoma in the human breast. J Natl Cancer Inst 50:1111–1118
Foreman DM, Bagley S, Moore J et al (1996) Three-dimensional analysis of the retinal vasculature using immunofluorescent staining and confocal laser scanning microscopy. Br J Ophthalmol 80:246–251
Salisbury JR, Deverell MH, Seaton JM et al (1997) Three-dimensional reconstruction of non-Hodgkin’s lymphoma in bone marrow trephines. J Pathol 181:451–454
Perez-Atayde AR, Sallan SE, Tedrow U et al (1997) Spectrum of tumor angiogenesis in the bone marrow of children with acute lymphoblastic leukaemia. Am J Pathol 150:815–821
Kay PA, Robb RA, Bostwick DG (1998) Prostate cancer microvessels: a novel method for three-dimensional reconstruction and analysis. Prostate 37:270–277
Duerstock BS, Bajaj CL, Pascucci V et al (2000) Advances in three-dimensional reconstruction of the experimental spinal cord injury. Comput Med Imaging Graph 24:389–406
Antiga L, Ene-Iordache B, Remuzzi G et al (2001) Automatic generation of glomerular capillary topological organization. Microvasc Res 62:346–354
Brey EM, King TW, Johnston C et al (2002) A technique for quantitative three-dimensional analysis of microvascular structure. Microvasc Res 63:279–294
Cassoni P, Gaetano L, Senetta R et al (2008) Histology far away from Flatland: 3D roller-coasting into grade-dependent angiogenetic patterns in oligodendrogliomas. J Cell Mol Med 12:564–568
Ohtake T, Abe R, Kimijima I et al (1995) Intraductal extension of primary invasive breast carcinoma treated by breast conservative surgery. Cancer 76:32–45
Ohtake T, Kimijima I, Fukushima T et al (2001) Computer-assisted complete three-dimensional reconstruction of the mammary ductal/lobular systems. Cancer 91:2263–2272
Going JJ, Moffat DF (2004) Escaping from flatland: clinical and biological aspects of human mammary duct anatomy in three dimensions. J Pathol 203:538–544
Marchiò C, Sapino A, Arisio R et al (2006) A new vision of tubular and tubulo-lobular carcinomas of the breast, as revealed by 3-D modelling. Histopathology 48:556–562
Papotti M, Manazza AD, Chiarle R et al (2004) Confocal microscope analysis and tridimensional reconstruction of papillary thyroid carcinoma nuclei. Virchows Arch 444:350–355
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Asioli, S., Eusebi, V., Gaetano, L. et al. The pre-lymphatic pathway, the rooths of the lymphatic system in breast tissue: a 3D study. Virchows Arch 453, 401–406 (2008). https://doi.org/10.1007/s00428-008-0657-y
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DOI: https://doi.org/10.1007/s00428-008-0657-y