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Claudin expression in Barrett's esophagus and adenocarcinoma

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Abstract

Claudins (CLDNs) are key molecules in cell adhesion, polarity, and control of paracellular solute transport. Several studies suggested that changes in claudin pattern have a role in cancer development. This study aimed to detect alterations in CLDN 1, 2, 3, 4, and 7 expression patterns in Barrett's esophagus (BE) and adenocarcinoma (ACC) compared with that in foveolar epithelium (FOV), normal squamous epithelium (SQ), and squamous cell carcinoma (SQCC). One hundred twenty five surgically or endoscopically removed, paraffin-embedded cases were studied by immunohistochemistry and analyzed statistically. BE, ACC, and FOV were dissected from 30 paraffin-embedded samples for further mRNA expression analysis. CLDN 7 was the dominating type in all epithelia and carcinomas, but its expression did not differ in normal and altered tissues. CLDN 1 expression was significantly increased in SQCC compared with that in SQ. CLDNs 3 and 4 were significantly elevated both in BE and ACC compared with that in FOV. CLDN 2 expression increased significantly in ACCs compared with that in BE. This is the first report proving similarities and differences regarding claudin expression pattern in BE and ACC compared with that in FOV and SQ. Our data prove a close link in CLDN pattern between BE and ACC, adding further evidence that BE is an alteration preceding esophageal ACC.

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Acknowledgements

This work was supported by grants NKFP-1A/0023/2002 from the Hungarian Ministry of Health, National Research Development Projects; OTKA T 049559 from the National Science Research Foundation; and ETT-077/2003 from the Hungarian Ministry of Health.

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Authors and Affiliations

  1. 2nd Department of Pathology, Semmelweis Medical University, Üllõi út 93, 1091, Budapest, Hungary

    Hajnalka Gyõrffy, Ágnes Holczbauer, Csilla Páska, Zsuzsa Schaff & András Kiss

  2. Department of Pathology, National Medical Centre, Szabolcs u. 35, 1135, Budapest, Hungary

    Pál Nagy

  3. Municipal Saint László Hospital, Gyáli út 5-7, 1097, Budapest, Hungary

    Zsuzsa Szabó

  4. 1st Department of Surgery, Semmelweis University, Üllõi út 78, 1082, Budapest, Hungary

    Péter Kupcsulik

  5. 1st Department of Internal Medicine, Semmelweis University, Korányi Sándor u. 2/a, 1083, Budapest, Hungary

    János Papp

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  1. Hajnalka Gyõrffy
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Correspondence to Zsuzsa Schaff.

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H. Gyõrffy and Á. Holczbauerc have equally contributed to this study

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Gyõrffy, H., Holczbauer, Á., Nagy, P. et al. Claudin expression in Barrett's esophagus and adenocarcinoma. Virchows Arch 447, 961–968 (2005). https://doi.org/10.1007/s00428-005-0045-9

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  • DOI: https://doi.org/10.1007/s00428-005-0045-9

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