Virchows Archiv

, Volume 446, Issue 3, pp 305–309 | Cite as

Immunohistochemical and cytogenetic characterization of acantholytic squamous cell carcinoma of the breast

  • Sebastian Aulmann
  • Philipp A. Schnabel
  • Birgit Helmchen
  • Hendrik Dienemann
  • Peter Drings
  • Herwart F. Otto
  • Hans Peter Sinn
Case Report

Abstract

Primary acantholytic squamous cell carcinoma (ASCC) of the breast is a rare and aggressive variant of invasive breast cancer. Here we report two new cases of ASCC and their immunohistochemical and cytogenetic characterization. One case was associated with systemic metastases and death and the other with local failure prior to loss of follow-up. Using comparative genomic hybridization (CGH), both tumors showed a high overall number of chromosomal imbalances with a similar pattern of gains and losses. Genetic aberrations common to both tumors included losses at 3p11-p25, 5q21-q31, 8p, 9, 13p13-q21, 16q12-q21, and 17p and gains at 1q31-qter, 7p, 18q12-qter, 19q, and 20. Immunohistochemically, the tumors were characterized by high proliferative activity, an uncommon cytokeratin expression profile, reduced E-cadherin staining, and overexpression of p53 and epithelial growth factor receptor (EGFR). The results of our analyses suggest that genetic alterations observed in ASCC of the breast include imbalances commonly observed in both mammary adenocarcinoma and squamous cell carcinoma of other locations. Furthermore, the overexpressed EGFR could be a possible therapeutic target for individual cases of this aggressive tumor type.

Keywords

Acantholytic squamous cell carcinoma Breast Comparative genomic hybridization Immunohistochemistry 

References

  1. 1.
    Aulmann S, Bläker H, Penzel R, Rieker RJ, Otto HF, Sinn HP (2003) CTCF gene mutations in invasive ductal breast cancer. Breast Cancer Res Treat 80:347–352CrossRefGoogle Scholar
  2. 2.
    Banerjee SS, Eyden BP, Wells S, McWilliam LJ, Harris M (1992) Pseudoangiosarcomatous carcinoma: a clinicopathological study of seven cases. Histopathol 21:13–23Google Scholar
  3. 3.
    Bayer-Garner IB, Smoller BR (2001) The expression of syndecan-1 is preferentially reduced compared with that of E-cadherin in acantholytic squamous cell carcinoma. J Cutan Pathol 28:83–89CrossRefGoogle Scholar
  4. 4.
    Eggers JW, Chesney TM (1984) Squamous cell carcinoma of the breast. Hum Pathol 15:526–531Google Scholar
  5. 5.
    Ellis IO, Schnitt SJ, Sastre-Garau X et al (2003) Invasive breast carcinoma. In: Tavassoli FA, Devilee P (eds) Pathology and genetics of tumours of the breast and female genital organs (World Health Organization classification of tumours). IARC Press, Lyon, pp 13–59Google Scholar
  6. 6.
    Eusebi V, Lamovec J, Cattani MG, Fedeli F, Millis RR (1986) Acantholytic variant of squamous-cell carcinoma of the breast. Am J Surg Pathol 10:855–861Google Scholar
  7. 7.
    Fisher ER, Palekar AS, Gregorio RM, Paulson JD (1983) Mucoepidermoid and squamous cell carcinomas of breast with reference to squamous metaplasia and giant cell tumors. Am J Surg Pathol 7:15–27Google Scholar
  8. 8.
    Gersell DJ, Katzenstein AL (1981) Spindle cell carcinoma of the breast. Hum Pathol 12:550–561Google Scholar
  9. 9.
    Handschuh G, Candidus S, Luber B, Reich U, Schott C, Oswald S, Becke H, Hutzler P, Birchmeier W, Hofler H, Becker KF (1999) Tumor-associated E-cadherin mutations alter cellular morphology, decrease cellular adhesion and increase cellular motility. Oncogene 18:4301–4312CrossRefGoogle Scholar
  10. 10.
    Isola J, DeVries S, Chu L, Ghazvini S, Waldman F (1994) Analysis of changes in DNA sequence copy number by comparative genomic hybridization in archival paraffin-embedded tumor samples. Am J Pathol 145:1301–1308Google Scholar
  11. 11.
    Korsching E, Packeisen J, Agelopoulos K, Eisenacher M, Voss R, Isola J, van Diest PJ, Brandt B, Boecker W, Buerger H (2002) Cytogenetic alterations and cytokeratin expression patterns in breast cancer: integrating a new model of breast differentiation into cytogenetic pathways of breast carcinogenesis. Lab Invest 82:1525–1533Google Scholar
  12. 12.
    Lever WF (1947) Adenoacanthoma of sweat glands: carcinoma of sweat glands with glandular and epidermal elements: report of four cases. Arch Dermatol Syphilol 56:157–171Google Scholar
  13. 13.
    Moisidis E, Ahmed S, Carmalt H, Gillett D (2002) Primary squamous cell carcinoma of the breast. ANZ J Surg 72:65–67CrossRefGoogle Scholar
  14. 14.
    Nakayama Y, Iwasaki H, Iwanaga S, Nakamura H, Shiroshita T, Kikuchi M, Jozaki H, Hashimoto M, Ikeda S (1997) Spindle cell carcinoma of the breast: a case report and an immunohistochemical study including p53 and Ki-67 expression. Pathol Int 47:404–411Google Scholar
  15. 15.
    Nappi O, Pettinato G, Wick MR (1989) Adenoid (acantholytic) squamous cell carcinoma of the skin. J Cutan Pathol 16:114–121Google Scholar
  16. 16.
    Parramore B, Hanly M, Yeh KA, McNeely T (1999) Acantholytic variant of squamous cell carcinoma of the breast: a case report. Am Surg 65:467–469Google Scholar
  17. 17.
    Petter G, Haustein UW (2000) Histologic subtyping and malignancy assessment of cutaneous squamous cell carcinoma. Dermatol Surg 26:521–530CrossRefGoogle Scholar
  18. 18.
    Rieker RJ, Joos S, Bartsch C, Willeke F, Schwarzbach M, Otano-Joos M, Ohl S, Hogel J, Lehnert T, Lichter P, Otto HF, Mechtersheimer G (2002) Distinct chromosomal imbalances in pleomorphic and in high-grade dedifferentiated liposarcomas. Int J Cancer 99:68–73CrossRefGoogle Scholar
  19. 19.
    Scarpatetti M, Tsybrovskyy O, Popper HH (2002) Cytokeratin typing as an aid in the differential diagnosis of primary versus metastatic lung carcinomas, and comparison with normal lung. Virchows Arch 440:70–76CrossRefGoogle Scholar
  20. 20.
    Toikkanen S (1981) Primary squamous cell carcinoma of the breast. Cancer 48:1629–1632Google Scholar
  21. 21.
    Vos CB, ter Haar NT, Rosenberg C, Peterse JL, Cleton-Jansen AM, Cornelisse CJ, van de Vijver MJ (1999) Genetic alterations on chromosome 16 and 17 are important features of ductal carcinoma in situ of the breast and are associated with histologic type. Br J Cancer 81:1410–1418CrossRefGoogle Scholar
  22. 22.
    Wargotz ES, Deos PH, Norris HJ (1989) Metaplastic carcinomas of the breast. II. Spindle cell carcinoma. Hum Pathol 20:732–740CrossRefGoogle Scholar
  23. 23.
    Yokoyama S, Yamakawa K, Tsuchiya E, Murata M, Sakiyama S, Nakamura Y (1992) Deletion mapping on the short arm of chromosome 3 in squamous cell carcinoma and adenocarcinoma of the lung. Cancer Res 52:873–877Google Scholar
  24. 24.
    Zoltan TB, Konick L, Coleman RJ (2001) Pure squamous cell carcinoma of the breast in a patient with previous adenocarcinoma of the breast: a case report and review of the literature. Am Surg 67:671–673Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Sebastian Aulmann
    • 1
  • Philipp A. Schnabel
    • 1
  • Birgit Helmchen
    • 1
  • Hendrik Dienemann
    • 2
  • Peter Drings
    • 2
  • Herwart F. Otto
    • 1
  • Hans Peter Sinn
    • 1
  1. 1.Department of PathologyUniversity of HeidelbergHeidelbergGermany
  2. 2.Thoraxklinik HeidelbergHeidelbergGermany

Personalised recommendations