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Difference in cytoplasmic localization pattern of neutral mucin among lobular endocervical glandular hyperplasia, adenoma malignum, and common adenocarcinoma of the uterine cervix

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Abstract

To investigate whether the cytoplasmic localization pattern of neutral mucin differs between lobular endocervical glandular hyperplasia (LEGH) and cervical adenocarcinoma (CxAd), including minimal-deviation adenocarcinoma (MDA), or adenoma malignum, alcian blue (pH 2.5)/periodic acid-Schiff (AB-PAS) staining was performed to formalin-fixed paraffin-embedded tissue sections of 13 lesions of LEGH and 53 tumors of CxAd, including 6 tumors of MDA. The cytoplasmic localization of neutral mucin was classified as a “whole cytoplasmic pattern,” in which neutral mucin filled the cytoplasm entirely, or as an “apical pattern,” in which neutral mucin was localized in the subsurface area only. Cytoplasmic neutral mucin patterns were detected in all 13 cases of LEGH and in 19 cases (36%) of CxAd, including five cases of MDA. The localization of neutral mucin was always the whole cytoplasmic pattern in 13 cases of LEGH, but was the apical pattern in these 19 cases of CxAd. The other 34 cases of CxAd, including 1 case of MDA, corresponded to the acid mucin pattern stained purple or blue or no staining pattern by AB-PAS. Among the 53 cases of CxAd, the apical neutral mucin pattern was an indicator of poorer patient prognosis by univariate and multivariate analyses. The examination of cytoplasmic localization of neutral mucin might be applicable, not only to differential diagnosis between LEGH and CxAd, including MDA, but also to estimate clinical aggressiveness of CxAd.

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Acknowledgments

The authors thank Professor Hironobu Sasano (Department of Pathology, Tohoku University Graduate School, Sendai, Miyagi, Japan), Professor Takashi Nakajima (Department of Pathology II, Gunma University School of Medicine, Maebashi, Gunma, Japan), and Professor Makio Mukai (Department of Diagnostic Pathology, Keio University Hospital, Tokyo, Japan) for providing tissue specimens and clinical data.

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Authors and Affiliations

  1. Department of Clinical Laboratory, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, 104-0045, Tokyo , Japan

    Isamu Hayashi & Tadakazu Shimoda

  2. Department of Pathology II, National Defense Medical College, 3-2 Namiki, Tokorozawa, 359-8513, Saitama , Japan

    Hitoshi Tsuda

  3. Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, 104-0045, Tokyo , Japan

    Arafumi Maeshima

  4. Department of Gynecological Oncology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, 104-0045, Tokyo , Japan

    Takahiro Kasamatsu, Takuro Yamada & Ryuichiro Tsunematsu

  5. Pathology Division, Shizuoka Cancer Center, 1007 Shimo-Nagakubo, Nagaizumi-cho, 411–8777, Shizuoka , Suntou-gun, Japan

    Isamu Hayashi

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  1. Isamu Hayashi
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  2. Hitoshi Tsuda
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  3. Tadakazu Shimoda
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  4. Arafumi Maeshima
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  5. Takahiro Kasamatsu
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  7. Ryuichiro Tsunematsu
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Correspondence to Isamu Hayashi.

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Hayashi, I., Tsuda, H., Shimoda, T. et al. Difference in cytoplasmic localization pattern of neutral mucin among lobular endocervical glandular hyperplasia, adenoma malignum, and common adenocarcinoma of the uterine cervix. Virchows Arch 443, 752–760 (2003). https://doi.org/10.1007/s00428-003-0899-7

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  • DOI: https://doi.org/10.1007/s00428-003-0899-7

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