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Vascular diffusion density and survival of patients with primary lung carcinomas

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Abstract

Aim

To investigate the clinical significance of tumour vascularisation in operated lung cancer patients.

Materials and methods

Histological slides obtained from 498 patients with potentially curative operated lung carcinomas in two different institutions of thoracic surgery were immunohistochemically stained with an anti-CD34 antibody and subjected to quantitative image analysis. Syntactic structure analysis measured the absolute and relative features of vessels, including the numerical tumour cells densities relative to their nearest neighbouring vessel. These data are associated with tumour volume, post-surgical TNM stage, and each patient's survival.

Results

The clinical data, including sex distribution, age of patients, pTNM stages and survival, did not differ between the two institutions. The tumour vascularisation (volume fraction, Vv) amounted to 7% in lung carcinomas, was independent from cell type and increased in advanced tumour stages (pT4, pN3). Advanced tumour stages presented with a higher numerical vascular density and with maintained minimum diameter and circumference of vessels. Each patient's survival was closely associated with the pN stage, tumour volume, cell type and numerical density of tumour cells within a distance less than 20 µm from the nearest neighbouring vessel due to multivariate statistical analysis.

Conclusion

Vascularisation of lung tumours becomes altered in advanced tumour stages. Of prognostic significance is the distribution of tumour cells in relation to the nearest neighbouring vessel only.

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Acknowledgement

The authors gratefully acknowledge the financial support of the Verein zur Förderung des biologisch–technologischen Fortschritts in der Medizin e.V.

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Authors and Affiliations

  1. Institute of Pathology, University Freiburg, Albertstrasse 19, 79104 , Freiburg, Germany

    Gian Kayser, Urs Riede & Martin Werner

  2. Institute of Pathology, Charité, Humboldt University, Berlin, Germany

    Jan Dirk Baumhäkel & Klaus Kayser

  3. Department of Thoracic Surgery, University Szeged, Szeged, Hungary

    Tamas Szöke & Imre Trojan

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  1. Gian Kayser
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  2. Jan Dirk Baumhäkel
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  3. Tamas Szöke
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  4. Imre Trojan
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  5. Urs Riede
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  6. Martin Werner
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  7. Klaus Kayser
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Correspondence to Gian Kayser.

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Kayser, G., Baumhäkel, J.D., Szöke, T. et al. Vascular diffusion density and survival of patients with primary lung carcinomas. Virchows Arch 442, 462–467 (2003). https://doi.org/10.1007/s00428-003-0791-5

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  • DOI: https://doi.org/10.1007/s00428-003-0791-5

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