Abstract
About 70% of zebrafish (Danio rerio) genes are orthologues of the human’s, which are of great interests, but still largely unknown for their functions. Recently, a report on human histone PARylation factor 1 (HPF1/C4orf27) showed that it is involved in DNA damage response along with poly (ADP-ribose) polymerase 1 (PARP1). However, its function in living organism remains unclear. Given that zebrafish has showed its values in modeling human diseases and physiology, we characterized a zebrafish homolog of human HPF1 by sequence alignment. We also analyzed its expression pattern during early development and among adult tissues. Furthermore, knocking down hpf1 by morpholinos affected zebrafish early development. Our work provides a novel clue for the mechanism of genome stability and early embryogenesis.
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Funding
This work was supported by National Key R&D Program of China (grant numbers 2017YFC1001901 and 2017YFC1001600), National Natural Science Foundation of China (31401223, 81330055); Guangdong Science and Technology Department Planning Project (2015A020212010); Zhujiang Program of Science and Technology Nova in Guangzhou (201710010042); and the Fundamental Research Funds for the Central Universities (Sun Yat-sen University, 161gpy30).
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Communicated by Caroline Brennan
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Zhang, Z., Sun, H., Chen, Y. et al. Analysis of hpf1 expression and function in early embryonic development of zebrafish. Dev Genes Evol 228, 141–147 (2018). https://doi.org/10.1007/s00427-018-0608-9
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DOI: https://doi.org/10.1007/s00427-018-0608-9