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Development Genes and Evolution

, Volume 224, Issue 4–6, pp 189–196 | Cite as

Characterization of high molecular weight glutenin subunit genes from the Ns genome of Psathyrostachys juncea

  • Lina Kong
  • Yu Liang
  • Lumin Qin
  • Lei Sun
  • Guangmin Xia
  • Shuwei Liu
Original Article

Abstract

The Ns genome of the genus Psathyrostachys possesses superior traits useful for wheat improvement. However, very little is known about the high molecular weight (HMW) subunits of glutenin encoded by the Ns genome. In this paper, we report the isolation of four alleles of HMW glutenin subunit gene from Psathyrostachys juncea. Sequence alignment data shows the four alleles have similar primary structure with those in wheat and other wheat-related grasses, with some unique modifications. All four sequences more closely resemble y-type, rather than x-type, glutenins. However, our results show three of the subunits (1Ns2-4) contain an extra glutamine residue in the N-terminal region not found on typical y-type subunits, as well as the x-type subunit specific sequence LAAQLPAMCRL. These three subunits likely represent an intermediate state in the divergence between x- and y-type subunits. Results also indicate that the Ns genome is more closely related to the St genome of Pseudoroegneria than any other Triticeae genomes.

Keywords

Glu-1 HMW-GS Ns genome Psathyrostachys Wheat processing quality 

Notes

Acknowledgments

This work was supported by funds from the Special Fund for Forest Scientific Research in the Public Welfare (No. 201204402), the Natural Science Foundation of China (No. 31271707; 31000568), and the Independent Innovation Foundation of Shandong University (2012TS009). We thank Dr. Austin Cape for careful reading and language editing.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Lina Kong
    • 1
  • Yu Liang
    • 2
  • Lumin Qin
    • 1
  • Lei Sun
    • 2
  • Guangmin Xia
    • 1
  • Shuwei Liu
    • 1
  1. 1.The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life SciencesShandong UniversityJinanPeople’s Republic of China
  2. 2.Shandong Academy of ForestryJinanPeople’s Republic of China

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