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Identification and developmental expression of leucine-rich repeat-containing G protein-coupled receptor 6 (lgr6) in the medaka fish, Oryzias latipes

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Abstract

G protein-coupled receptors are critical regulators of diverse developmental processes such as oocyte maturation, fertilization, gastrulation, and organogenesis. To further study the molecular mechanisms underlying these processes, we cloned and characterized the orphan leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6), a stem cell marker in mammalian hair follicles, in medaka fish, Oryzias latipes. To examine the expression pattern of lgr6, we performed whole-mount in situ hybridization (WISH) during embryogenesis. The expression of lgr6 was first detected as a band in the anterior part of the posterior brain vesicle in 0.5–1 day post fertilization (dpf) embryos. This band disappeared by 2 dpf, but new signals appeared in the otic vesicles bordering the original band and also detected in the nasal placode and posterior lateral line primordia. At later stages (3–5 dpf), lgr6 was widely expressed in the brain, otic vesicle, neuromasts, root of the pectoral fin, cranial cartilage, and gut. Then, we conducted more detailed expression analysis of lgr6 in adult gut using WISH and immunohistochemical staining. Lgr6-positive cells were detected in the crypt-like proliferative zone and in parts of the villus. We also performed RT-PCR of mRNAs from different tissues. The lgr6 mRNA was found highest in the kidney and gill. The transcript was also present in the brain, heart, liver, spleen, intestine, skeletal muscle, testis, and ovary, similar to that of mammalian LGR6. These results suggest that medaka lgr6 plays an important role in organ development during embryogenesis and serves as a good molecular marker for future studies of postembryonic organ-specific development in mammals.

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Acknowledgments

We thank Y. Ishikawa for providing Hd-rR and Y. Wakamatsu for providing STII through the National BioResource Project. We also thank Y. Jyosaki for maintaining the fish and sectioning, K. Shizuma and Y. Nogami for help with RT-PCR.

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Authors and Affiliations

  1. National Institute of Advanced Industrial Science and Technology, Amagasaki, Hyogo, 661-0974, Japan

    Tomonori Deguchi, Takashi Kawasaki, Hiroe Ohnishi & Shunsuke Yuba

  2. Suntory Foundation for Life Sciences, Bioorganic Research Institute, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, 618-8503, Japan

    Toshio Takahashi

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  1. Tomonori Deguchi
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  2. Takashi Kawasaki
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  3. Hiroe Ohnishi
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  4. Shunsuke Yuba
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  5. Toshio Takahashi
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Correspondence to Toshio Takahashi.

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Communicated by: M. Hammerschmidt

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Deguchi, T., Kawasaki, T., Ohnishi, H. et al. Identification and developmental expression of leucine-rich repeat-containing G protein-coupled receptor 6 (lgr6) in the medaka fish, Oryzias latipes . Dev Genes Evol 222, 217–227 (2012). https://doi.org/10.1007/s00427-012-0403-y

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  • DOI: https://doi.org/10.1007/s00427-012-0403-y

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