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Thyroid hormone-regulated expression of nuclear lamins correlates with dedifferentiation of intestinal epithelial cells during Xenopus laevis metamorphosis

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Abstract

In the Xenopus laevis intestine during metamorphosis, which is triggered by thyroid hormone (TH), the adult epithelium develops and replaces the larval one undergoing apoptosis. We have previously shown that progenitor/stem cells of the adult epithelium originate from some differentiated larval epithelial cells. To investigate molecular mechanisms underlying larval epithelial dedifferentiation into the adult progenitor/stem cells, we here focused on nuclear lamin A (LA) and lamin LIII (LIII), whose expression is generally known to be correlated with the state of cell differentiation. We analyzed the spatiotemporal expression of LA and LIII during X. laevis intestinal remodeling by reverse transcription PCR, Western blotting, and immunohistochemistry. At the onset of natural metamorphosis, when the adult epithelial progenitor cells appear as small islets, the expression of LA is down-regulated, but that of LIII is up-regulated only in the islets. Then, as the adult progenitor cells differentiate, the expression of LA is up-regulated, whereas that of LIII is down-regulated in the adult cells. As multiple intestinal folds form, adult epithelial cells positive for LIII become restricted only to the troughs of the folds. In addition, we have shown that TH up- or down-regulates the expression of these lamins in the premetamorphic intestine as during natural metamorphosis. These results indicate that TH-regulated expression of LA and LIII closely correlates with dedifferentiation of the epithelial cells in the X. laevis intestine, suggesting the involvement of the lamins in the process of dedifferentiation during amphibian metamorphosis.

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Acknowledgments

We would like to thank Drs. Masakazu Fujiwara (Nippon Medical School), Itaru Hasunuma (Waseda Univ), and Kosuke Kawamura (Waseda Univ) for providing us technical information. This work was supported in part by JSPS Grants-in-Aid for Scientific Research (C) to A. I.-O (grant no. 20570060).

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Authors and Affiliations

  1. Department of Biology, Nippon Medical School, 2-297-2 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa, 211-0063, Japan

    Takashi Hasebe & Atsuko Ishizuya-Oka

  2. Department of Molecular Biology, Institute of Development and Aging Sciences, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa, 211-8533, Japan

    Mitsuko Kajita & Atsuko Ishizuya-Oka

  3. Laboratory of Molecular Genetics, Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan

    Mari Iwabuchi & Keita Ohsumi

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  1. Takashi Hasebe
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Correspondence to Atsuko Ishizuya-Oka.

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Communicated by T. Hollemann

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Hasebe, T., Kajita, M., Iwabuchi, M. et al. Thyroid hormone-regulated expression of nuclear lamins correlates with dedifferentiation of intestinal epithelial cells during Xenopus laevis metamorphosis. Dev Genes Evol 221, 199–208 (2011). https://doi.org/10.1007/s00427-011-0371-7

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